Difference between revisions of "Part:BBa K3738013:Design"

 
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===Source===
 
===Source===
  
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BBa_J64997, BBa_B0034, BBa_K3738003, BBa_B0015
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Ms2 Bacteriophage
  
 
===References===
 
===References===
 +
[1] Glasgow, J., Capehart, S., Francis, M., and Tullman-Ercek, D. (2012) Osmolyte-Mediated Encapsulation of Proteins inside MS2 viral capsids. ACSNAno. 6, 8658-8664
 +
<br>
 +
[2] Vives, E., Schmidt, J., And Pelegrin, A. (2008) Cell penetrating and cell-targeting peptides in drug delivery. Biochimica et biophysica Acta. 1786, 126-138

Latest revision as of 03:38, 22 October 2021


MS2 Bacteriophage Maturation Protein A with N-Terminal Histidine Tag and TEV Site (Composite)


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 646
    Illegal BamHI site found at 920
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 1009


Design Notes

The coding region in the part has been codon optimized for use in E.coli.


Source

Ms2 Bacteriophage

References

[1] Glasgow, J., Capehart, S., Francis, M., and Tullman-Ercek, D. (2012) Osmolyte-Mediated Encapsulation of Proteins inside MS2 viral capsids. ACSNAno. 6, 8658-8664
[2] Vives, E., Schmidt, J., And Pelegrin, A. (2008) Cell penetrating and cell-targeting peptides in drug delivery. Biochimica et biophysica Acta. 1786, 126-138