Difference between revisions of "Part:BBa K3844001:Sequence, Features, and Subparts"
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==1. Function== | ==1. Function== | ||
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CCCTC-binding factor, otherwise known as (CTCF), is a transcription factor. It has numerous functions in synthetic biology, including but not limited to functioning as a transcriptional activator or an insulator protein. CTCF transcription factors can also call for other transcription factors while bound to these important entities known as “chromatin domain boundaries.” | CCCTC-binding factor, otherwise known as (CTCF), is a transcription factor. It has numerous functions in synthetic biology, including but not limited to functioning as a transcriptional activator or an insulator protein. CTCF transcription factors can also call for other transcription factors while bound to these important entities known as “chromatin domain boundaries.” | ||
| + | |||
| + | ==2. Design Consideration== | ||
| + | Our project requires for the transcription factor to be dependent on the presence of VOCs. When researching the design of trimethylbenzene, the VOC that this transcription factor binds to, we needed to make sure that, hypothetically, everything would work based on our model. After checking through extensive research that our design would fit with this transcription factor, we chose it. | ||
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| + | ==3. Characterization== | ||
| + | Sequence: GGAGGCCCTCCCACTCTCCTCTCGCACCCGCTTTTAGG | ||
| + | TGGCCCTCTTCTAGGGCGGAGAGATCACCAAAAGCCGCTGAGCAAGGA | ||
| + | CAGCCCTTCCAGTCGTCGCTCGCGGGCACGGAGCTGGGAGCGCGCGGG | ||
| + | AACCGACCGGGCCTTACTCTGGGGCGGGCAGCTGTAAGACATTCGGAG | ||
| + | GGCGGGTCGGGCCTGGCTGGGGCGGAGACTGGGCGGGGCCGGGCCCGG | ||
| + | GAGCCTCCTCAGCCCCACTGGCTTGCCCCGCGCGCGCTTCCTCCAGCC | ||
| + | ACCCCCACTTCCCCAACCCTGTACGGGTTCAGGGGGCCGGAGACAGCA | ||
| + | GCTAGGGAATAAGGTCAAGCGGACTGGATCCGGCACCGCCCAGGGGCG | ||
| + | GCGGCCTCCGCCCAGGAAGCCCCACCCAGCGGGAGCCAGGACGCCGCG | ||
| + | GCGCCGGCAGAGCCCACGAGCGACCCGGCGAGGGCGCGTGCGGCGTGA | ||
| + | CGACGCGACTCGCGGCGCGTCCCTGACGCCATCGCGCCCGCTTTTGAA | ||
| + | AGTTGGCGCCCGGGGCCGCCTCCCATGCTGCCCTGCGCCAACCCCTCC | ||
| + | CCTCACCTTTCCTCCCCCGCCCTCTACTCGCCGCCCGGCCGGTCCCCC | ||
| + | CACCCGTCCCTTCCCTTATCAGCACCCGCGGCCCCGGCAGCGCCGACG | ||
| + | CAGGCGCACTGCACCGCGCCGCCGCCATTTTGTGTCTGAGCCTGTGGA | ||
| + | GCGATTAAACCGTGCGCGGAGCTGCTTCTTTGGCGGCAGCGGCGGCGG | ||
| + | CGGTGGCCGGTGCGGACGCGCGGAGCTCGCCGGAGACGCCGGGTGGCC | ||
| + | GGAGCCGTGGAGCGGCGGCGGAGCGGGCGCCGCGGGGGGTGTGGCGCG | ||
| + | GAG | ||
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| + | ==4. References== | ||
| + | 1. https://www.sciencedirect.com/topics/biochemistry-genetics-and-molecular-biology/transcription-factor-ctcf | ||
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| + | 2. Fu Y, Sinha M, Peterson CL, Weng Z (2008). "The insulator binding protein CTCF positions 20 nucleosomes around its binding sites across the human genome". PLOS Genetics. 4 (7): e1000138. doi:10.1371/journal.pgen.1000138. PMC 2453330. PMID 18654629. | ||
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| + | 3. https://pubchem.ncbi.nlm.nih.gov/compound/1_2_4-Trimethylbenzene | ||
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| + | 4. https://www.researchgate.net/publication/319293199_CCCTC-binding_factor_is_essential_to_the_maintenance_and_quiescence_of_hematopoietic_stem_cells_in_mice | ||
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| + | <!-- --> | ||
| + | <span class='h3bb'>Sequence and Features</span> | ||
| + | <partinfo>BBa_K3844001 SequenceAndFeatures</partinfo> | ||
Latest revision as of 03:38, 22 October 2021
1. Function
CCCTC-binding factor, otherwise known as (CTCF), is a transcription factor. It has numerous functions in synthetic biology, including but not limited to functioning as a transcriptional activator or an insulator protein. CTCF transcription factors can also call for other transcription factors while bound to these important entities known as “chromatin domain boundaries.”
2. Design Consideration
Our project requires for the transcription factor to be dependent on the presence of VOCs. When researching the design of trimethylbenzene, the VOC that this transcription factor binds to, we needed to make sure that, hypothetically, everything would work based on our model. After checking through extensive research that our design would fit with this transcription factor, we chose it.
3. Characterization
Sequence: GGAGGCCCTCCCACTCTCCTCTCGCACCCGCTTTTAGG TGGCCCTCTTCTAGGGCGGAGAGATCACCAAAAGCCGCTGAGCAAGGA CAGCCCTTCCAGTCGTCGCTCGCGGGCACGGAGCTGGGAGCGCGCGGG AACCGACCGGGCCTTACTCTGGGGCGGGCAGCTGTAAGACATTCGGAG GGCGGGTCGGGCCTGGCTGGGGCGGAGACTGGGCGGGGCCGGGCCCGG GAGCCTCCTCAGCCCCACTGGCTTGCCCCGCGCGCGCTTCCTCCAGCC ACCCCCACTTCCCCAACCCTGTACGGGTTCAGGGGGCCGGAGACAGCA GCTAGGGAATAAGGTCAAGCGGACTGGATCCGGCACCGCCCAGGGGCG GCGGCCTCCGCCCAGGAAGCCCCACCCAGCGGGAGCCAGGACGCCGCG GCGCCGGCAGAGCCCACGAGCGACCCGGCGAGGGCGCGTGCGGCGTGA CGACGCGACTCGCGGCGCGTCCCTGACGCCATCGCGCCCGCTTTTGAA AGTTGGCGCCCGGGGCCGCCTCCCATGCTGCCCTGCGCCAACCCCTCC CCTCACCTTTCCTCCCCCGCCCTCTACTCGCCGCCCGGCCGGTCCCCC CACCCGTCCCTTCCCTTATCAGCACCCGCGGCCCCGGCAGCGCCGACG CAGGCGCACTGCACCGCGCCGCCGCCATTTTGTGTCTGAGCCTGTGGA GCGATTAAACCGTGCGCGGAGCTGCTTCTTTGGCGGCAGCGGCGGCGG CGGTGGCCGGTGCGGACGCGCGGAGCTCGCCGGAGACGCCGGGTGGCC GGAGCCGTGGAGCGGCGGCGGAGCGGGCGCCGCGGGGGGTGTGGCGCG GAG
4. References
2. Fu Y, Sinha M, Peterson CL, Weng Z (2008). "The insulator binding protein CTCF positions 20 nucleosomes around its binding sites across the human genome". PLOS Genetics. 4 (7): e1000138. doi:10.1371/journal.pgen.1000138. PMC 2453330. PMID 18654629.
3. https://pubchem.ncbi.nlm.nih.gov/compound/1_2_4-Trimethylbenzene
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 352
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 425
- 1000COMPATIBLE WITH RFC[1000]