Difference between revisions of "Part:BBa K3771041"

Latest revision as of 03:35, 22 October 2021

CDO1-6xHis

Description

L-cysteine dioxygenase (CDO1) is an enzyme that weighs 23 kDa. CDO1 functions in the L-cysteine sulfinic acid taurine biosynthesis pathway, converting L-cysteine to L-cysteine sulfinic acid. [1]

Fig. 1. Taurine pathways in E. coli [1,2].

CDO1 is part of the L-cysteine sulfinic acid pathway, one of three possible taurine synthesis pathways. CDO1 oxidizes L-cysteine naturally found in the intestine into L-cysteine sulfinic acid, which is later converted to taurine by CSAD [1].

Usage

CDO1 was used in in vivo testing of taurine production. The sequence for CDO1 enzyme and trc promoter were ligated and transformed into E. coli to calculate taurine production using high-performance liquid chromatography (HPLC).

Characterization

Fig. 2. PCR confirmation of cdo1-6xHis

CDO1 was used in in vivo testing of taurine production. The sequence for CDO1 enzyme and trc promoter were ligated and transformed into E. coli to calculate taurine production using high-performance liquid chromatography (HPLC). A 6xHis-tag is added to the C-terminal of the CDO1 protein, allowing for confirmation of CDO1 expression by western blot using the anti-6xHis antibody.

References
1. Joo Y-C, Ko YJ, You SK, et al. Creating a New Pathway in Corynebacterium glutamicum for the Production of Taurine as a Food Additive. Journal of Agricultural and Food Chemistry. 2018;66(51):13454-13463. doi:10.1021/acs.jafc.8b05093

Sequence and Features