Difference between revisions of "Part:BBa K3739003"

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===Usage===
 
===Usage===
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Used to construct the expression system and obtained the composite part BBa_K3739043.  
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Used to construct the expression system and obtained the composite part <partinfo>BBa_K3739043</partinfo>.  
  
 
===Characterization===
 
===Characterization===
 
1.Colony PCR
 
1.Colony PCR
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We construct his-GFP CDS with J23100 promotor and B0030 RBS, the transformed cells are selected by colony PCR. The experiment result is showed in Fig. 1.  
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We construct <partinfo>BBa_K3739003</partinfo> with J23100 promotor, B0030 RBS and B0010, the transformed cells are selected by colony PCR. The experiment result is showed in Fig. 1. <br/>
 
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[[File:T--XMU-China--his-GFP.png|200px|alt text]]
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<center>[[File:T--XMU-China--his-GFP.png|200px|alt text]]</center><br/>
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Fig. 1. DNA gel electrophoresis of colony PCR product of J23100-B0030-his-GFP-pSB1C3.  
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'''Fig. 1.''' DNA gel electrophoresis of colony PCR product of J23100-B0030-his-GFP-pSB1C3.<br/>
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<br/>
 
<span class='h3bb'>Sequence and Features</span>
 
<span class='h3bb'>Sequence and Features</span>
 
<partinfo>BBa_K3739003 SequenceAndFeatures</partinfo>
 
<partinfo>BBa_K3739003 SequenceAndFeatures</partinfo>

Latest revision as of 03:31, 22 October 2021


his-GFP

his-tag is used to be detected by anti-his antibody and purify the his-linking fusion, GFP can emit green fluorescence.

Biology

GFP Protein tags are peptides genetically fused to the proteins of interest. There are many different kinds of tags developed for for different purposes. For labeling experiments, normally the proteins of interest are tagged directly by fluorescent tags. Green fluorescent protein (GFP) make it easier to study protein localization, interactions and dynamics when fusing with other peptides and proteins.

Usage

Used to construct the expression system and obtained the composite part BBa_K3739043.

Characterization

1.Colony PCR We construct BBa_K3739003 with J23100 promotor, B0030 RBS and B0010, the transformed cells are selected by colony PCR. The experiment result is showed in Fig. 1.

alt text

Fig. 1. DNA gel electrophoresis of colony PCR product of J23100-B0030-his-GFP-pSB1C3.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 73