Difference between revisions of "Part:BBa K3738008:Design"

(→‎Source)
(→‎Design Notes)
 
(One intermediate revision by the same user not shown)
Line 7: Line 7:
  
 
===Design Notes===
 
===Design Notes===
−
This part is an improved version of the part BBa_K2683012, and has been modified to include an N-terminal histidine tag and has had the polyarginine tag removed.
 
  
 
This part has not yet been codon-optimized.
 
This part has not yet been codon-optimized.
Line 17: Line 16:
 
===References===
 
===References===
 
de Martín Garrido, N., Crone, M.A., Ramlaul, K., Simpson, P.A., Freemont, P.S. and Aylett, C.H.S. (2020), Bacteriophage MS2 displays unreported capsid variability assembling T = 4 and mixed capsids. Mol Microbiol, 113: 143-152. https://doi.org/10.1111/mmi.14406
 
de Martín Garrido, N., Crone, M.A., Ramlaul, K., Simpson, P.A., Freemont, P.S. and Aylett, C.H.S. (2020), Bacteriophage MS2 displays unreported capsid variability assembling T = 4 and mixed capsids. Mol Microbiol, 113: 143-152. https://doi.org/10.1111/mmi.14406
 +
 +
[1] Glasgow, J., Capehart, S., Francis, M., and Tullman-Ercek, D. (2012) Osmolyte-Mediated Encapsulation of Proteins inside MS2 viral capsids. ACSNAno. 6, 8658-8664
 +
 +
[2] Vives, E., Schmidt, J., And Pelegrin, A. (2008) Cell penetrating and cell-targeting peptides in drug delivery. Biochimica et biophysica Acta. 1786, 126-138
 +
 +
https://www.uniprot.org/uniprot/P03612

Latest revision as of 03:30, 22 October 2021


MS2 Bacteriophage Capsid Protein Dimer


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 138
    Illegal PstI site found at 546
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 138
    Illegal PstI site found at 546
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 138
    Illegal PstI site found at 546
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 138
    Illegal PstI site found at 546
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

This part has not yet been codon-optimized.

Source

This part comes from the MS2 bacteriophage.

References

de Martín Garrido, N., Crone, M.A., Ramlaul, K., Simpson, P.A., Freemont, P.S. and Aylett, C.H.S. (2020), Bacteriophage MS2 displays unreported capsid variability assembling T = 4 and mixed capsids. Mol Microbiol, 113: 143-152. https://doi.org/10.1111/mmi.14406

[1] Glasgow, J., Capehart, S., Francis, M., and Tullman-Ercek, D. (2012) Osmolyte-Mediated Encapsulation of Proteins inside MS2 viral capsids. ACSNAno. 6, 8658-8664

[2] Vives, E., Schmidt, J., And Pelegrin, A. (2008) Cell penetrating and cell-targeting peptides in drug delivery. Biochimica et biophysica Acta. 1786, 126-138

https://www.uniprot.org/uniprot/P03612