Difference between revisions of "Part:BBa K3738006:Design"

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===Design Notes===
 
===Design Notes===
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This part is an improved version of the part BBa_K2683012, and has been modified to include an N-terminal histidine tag and has had the polyarginine tag removed.
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This part is an improved version of the part BBa_K2683012, and has been modified to include an C-terminal histidine tag and has had the polyarginine tag removed.
 
This part has been codon optimized for use in E.coli.
 
This part has been codon optimized for use in E.coli.
  
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===References===
 
===References===
 
[1] Glasgow, J., Capehart, S., Francis, M., and Tullman-Ercek, D. (2012) Osmolyte-Mediated Encapsulation of Proteins inside MS2 viral capsids. ACSNAno. 6, 8658-8664
 
[1] Glasgow, J., Capehart, S., Francis, M., and Tullman-Ercek, D. (2012) Osmolyte-Mediated Encapsulation of Proteins inside MS2 viral capsids. ACSNAno. 6, 8658-8664
 +
 
[2] Vives, E., Schmidt, J., And Pelegrin, A. (2008) Cell penetrating and cell-targeting peptides in drug delivery. Biochimica et biophysica Acta. 1786, 126-138
 
[2] Vives, E., Schmidt, J., And Pelegrin, A. (2008) Cell penetrating and cell-targeting peptides in drug delivery. Biochimica et biophysica Acta. 1786, 126-138
 +
 
https://www.uniprot.org/uniprot/P03612
 
https://www.uniprot.org/uniprot/P03612

Latest revision as of 03:27, 22 October 2021


MS2 Bacteriophage Capsid Protein with C-Terminal Histidine Tag


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

This part is an improved version of the part BBa_K2683012, and has been modified to include an C-terminal histidine tag and has had the polyarginine tag removed. This part has been codon optimized for use in E.coli.

Source

This part comes from the MS2 bacteriophage.

References

[1] Glasgow, J., Capehart, S., Francis, M., and Tullman-Ercek, D. (2012) Osmolyte-Mediated Encapsulation of Proteins inside MS2 viral capsids. ACSNAno. 6, 8658-8664

[2] Vives, E., Schmidt, J., And Pelegrin, A. (2008) Cell penetrating and cell-targeting peptides in drug delivery. Biochimica et biophysica Acta. 1786, 126-138

https://www.uniprot.org/uniprot/P03612