Difference between revisions of "Part:BBa K3771020"

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	<img src="https://2021.igem.org/wiki/images/c/c9/T--NCKU_Tainan--taurine_pathway_1.png" style="width:60%;">		<img src="https://2021.igem.org/wiki/images/c/c9/T--NCKU_Tainan--taurine_pathway_1.png" style="width:60%;">
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−	<p align="center">Fig.1 Taurine pathways in <i>E. coli</i></p>	+	<p align="center">Fig. 1. Taurine pathways in <i>E. coli</i></p>
	<br><b style="font-size:1.3rem">Usage</b>		<br><b style="font-size:1.3rem">Usage</b>
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	<br><b style="font-size:1.3rem">Characterization</b>		<br><b style="font-size:1.3rem">Characterization</b>
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−	<img src="https://2021.igem.org/wiki/images/e/e0/T--NCKU_Tainan--colony_pcr_pspA_JJU10.jpg	+	<img src="https://2021.igem.org/wiki/images/f/fa/T--NCKU_Tainan--colony_jju.jpg
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−	<p align="center">圖片描述</p>	+	<p align="center">Fig. 2. Colony PCR confirmation of the construction</p>
−	<br><b style="font-size:1.3rem">Reference</b>	+	<br><b style="font-size:1.3rem">Reference</b><br>
	<br>1. Darwin AJ. The phage-shock-protein response. Molecular Microbiology. 2005;57(3):621-628. doi:10.1111/j.1365-2958.2005.04694.x		<br>1. Darwin AJ. The phage-shock-protein response. Molecular Microbiology. 2005;57(3):621-628. doi:10.1111/j.1365-2958.2005.04694.x
	<a href="https://pubmed.ncbi.nlm.nih.gov/16045608/" alt="" target="_blank">https://pubmed.ncbi.nlm.nih.gov/16045608/</a>		<a href="https://pubmed.ncbi.nlm.nih.gov/16045608/" alt="" target="_blank">https://pubmed.ncbi.nlm.nih.gov/16045608/</a>
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	<!-- Add more about the biology of this part here		<!-- Add more about the biology of this part here
	===Usage and Biology===		===Usage and Biology===

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Latest revision as of 03:21, 22 October 2021

PpspA-JJU

Description

This composite part is a component of the IFN-γ sensing system and was used to express the taurine production enzyme, JJU.

Biology

Binding of IFN-γ to the OmpA/OprF chimeric protein induces the response of the phage shock protein (Psp) system, a highly conserved stress response system in enterobacteria.[1]Signal transduction from the outer membrane to the inner membrane activates the pspA promoter, initiating expression of JJU. JJU converts L-cysteine into L-cystate in the taurine synthesis L-cystate pathway.

Fig. 1. Taurine pathways in E. coli

Usage

We ligased the jju fragment and pspA promoter on the pSU expression vector and transformed it into DH5α to complete construction of the plasmid.

Characterization

Fig. 2. Colony PCR confirmation of the construction

Reference

1. Darwin AJ. The phage-shock-protein response. Molecular Microbiology. 2005;57(3):621-628. doi:10.1111/j.1365-2958.2005.04694.x https://pubmed.ncbi.nlm.nih.gov/16045608/
Sequence and Features