Difference between revisions of "Part:BBa K3771022"
 
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<br><b style="font-size:1.3rem">Description</b>
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<br><b style="font-size:1.3rem">Description</b><br>
 
   <br>This composite part is a component of the IFN-γ sensing system and was used to express the taurine production enzyme, CoaBC. <br>
 
   <br>This composite part is a component of the IFN-γ sensing system and was used to express the taurine production enzyme, CoaBC. <br>
 
    
 
    
  
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  <br><b style="font-size:1.3rem">Biology</b>
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  <br><b style="font-size:1.3rem">Biology</b><br>
 
   <br>Binding of IFN-γ to the OmpA/OprF chimeric protein induces the response of the phage shock protein (Psp) system, a highly conserved stress response system in enterobacteria. <a href="https://pubmed.ncbi.nlm.nih.gov/16045608/
 
   <br>Binding of IFN-γ to the OmpA/OprF chimeric protein induces the response of the phage shock protein (Psp) system, a highly conserved stress response system in enterobacteria. <a href="https://pubmed.ncbi.nlm.nih.gov/16045608/
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" alt="" target="_blank">[3]</a> Signal transduction from the outer membrane to the inner membrane activates the pspA promoter, initiating expression of CoaBC. CoaBC converts L-cystate into taurine in the taurine synthesis L-cystate pathway.
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" alt="" target="_blank">[1]</a> Signal transduction from the outer membrane to the inner membrane activates the pspA promoter, initiating expression of CoaBC. CoaBC converts L-cystate into taurine in the taurine synthesis L-cystate pathway.
 
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<div style="width=100%; display:flex; align-items: center; justify-content: center;">
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<img src="https://2021.igem.org/wiki/images/c/c9/T--NCKU_Tainan--taurine_pathway_1.png" style="width:60%;">
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<p align="center">Fig. 1. Taurine pathways in <i>E. coli</i></p>
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  <br><b style="font-size:1.3rem">Usage</b><br>
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  <br>We ligased the <i>coaBC</i> fragment and <i>pspA</i> promoter on the pUC expression vector and transformed it into DH5α to complete construction of the plasmid. <br>
  
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  <br><b style="font-size:1.3rem">Usage</b>
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<br><b style="font-size:1.3rem">Characterization</b><br>
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  <br>We ligased the <i>coaBC</i> fragment and <i>pspA</i> promoter on the pSU expression vector and transformed it into DH5α to complete construction of the plasmid. <br>
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<br><b style="font-size:1.3rem">Characterization</b>
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<div style="width=100%; display:flex; align-items: center; justify-content: center;">
 
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<img src="https://2021.igem.org/wiki/images/c/c5/T--NCKU_Tainan--colony_pcr_pspA_CoaBC.jpg
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<img src="https://2021.igem.org/wiki/images/4/46/T--NCKU_Tainan--colony_coaBC.jpg
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<p align="center">圖片描述</p>
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<p align="center">Fig. 2. Colony PCR confirmation of the construction</p>
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<br><b style="font-size:1.3rem">Reference</b>
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  <br>3. Darwin AJ. The phage-shock-protein response. Molecular Microbiology. 2005;57(3):621-628. doi:10.1111/j.1365-2958.2005.04694.x<a href="https://pubmed.ncbi.nlm.nih.gov/16045608/" alt="" target="_blank">https://pubmed.ncbi.nlm.nih.gov/16045608/</a><br>
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<br><b style="font-size:1.3rem">Reference</b><br>
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  <br>1. Darwin AJ. The phage-shock-protein response. Molecular Microbiology. 2005;57(3):621-628. doi:10.1111/j.1365-2958.2005.04694.x<a href="https://pubmed.ncbi.nlm.nih.gov/16045608/" alt="" target="_blank">https://pubmed.ncbi.nlm.nih.gov/16045608/</a><br>
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<!-- Add more about the biology of this part here
 
<!-- Add more about the biology of this part here
 
===Usage and Biology===
 
===Usage and Biology===

Latest revision as of 03:21, 22 October 2021


PpspA-CoaBC


Description

This composite part is a component of the IFN-γ sensing system and was used to express the taurine production enzyme, CoaBC.

Biology

Binding of IFN-γ to the OmpA/OprF chimeric protein induces the response of the phage shock protein (Psp) system, a highly conserved stress response system in enterobacteria. [1] Signal transduction from the outer membrane to the inner membrane activates the pspA promoter, initiating expression of CoaBC. CoaBC converts L-cystate into taurine in the taurine synthesis L-cystate pathway.

Fig. 1. Taurine pathways in E. coli


Usage

We ligased the coaBC fragment and pspA promoter on the pUC expression vector and transformed it into DH5α to complete construction of the plasmid.

Characterization

Fig. 2. Colony PCR confirmation of the construction


Reference

1. Darwin AJ. The phage-shock-protein response. Molecular Microbiology. 2005;57(3):621-628. doi:10.1111/j.1365-2958.2005.04694.xhttps://pubmed.ncbi.nlm.nih.gov/16045608/
Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 12
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 727
  • 1000
    COMPATIBLE WITH RFC[1000]