Difference between revisions of "Part:BBa K3771076"
 
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<img src="https://2021.igem.org/wiki/images/c/c9/T--NCKU_Tainan--taurine_pathway_1.png" style="width:50%;">
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<p align="center">Fig. 1 L-cysteine sulfonic acid synthase catalyzes the reaction that turns O-phospho-L-serine into L-cysteine sulfonic acid. CDO1, L-cysteine dioxygenase; CSAD, L-cysteine sulfinic acid decarboxylase</p>
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  <p align="center">Fig. 1. L-cysteine sulfonic acid synthase catalyzes the reaction that turns O-phospho-L-serine into L-cysteine sulfonic acid. CDO1, L-cysteine dioxygenase; CSAD, L-cysteine sulfinic acid decarboxylase</p>
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   <br>pSAA-<i>P<sub>LacI</sub>-csd</i><br>   
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   <br>pSAA-<i>P<sub>lacI</sub>-csad</i><br>   
 
    
 
    
 
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<img src="https://2021.igem.org/wiki/images/8/86/T--NCKU_Tainan--CSAD-PCR.png" style="width:35%;">
 
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   <p align="center">Fig. 2 Confirmation of <i>csad</i> fragment by PCR. M: Marker; Lane 1: <i>csad</i> (1368 bp)</p>
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   <p align="center">Fig. 2. Confirmation of <i>csad</i> fragment by PCR. M: Marker; Lane 1: <i>csad</i> (1368 bp)</p>
 
    
 
    
 
    
 
    
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<img src="https://2021.igem.org/wiki/images/4/48/T--NCKU_Tainan--CSAD1-Vactor-digestion.png" style="width:35%;">
 
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<p align="center">Fig. 3 Confirmation of pSAA fragment by digestion. M: Marker; Lane 1: pSAA (2531 bp)</p>
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<p align="center">Fig. 3. Confirmation of pSAA fragment by digestion. M: Marker; Lane 1: pSAA (2531 bp)</p>
  
 
    
 
    

Latest revision as of 03:17, 22 October 2021


PlacI-CSAD


Description

PlacI-csad is a composite part consisting of the lacI promoter and the csad sequences. This part was used in in vivo testing of taurine production. The sequence for csad enzyme and lacI promoter were ligated and transformed into E. coli to calculate taurine production using high-performance liquid chromatography (HPLC).

Biology

lacI promoter constitutively facilitates the expression of CSAD enzyme.

Fig. 1. L-cysteine sulfonic acid synthase catalyzes the reaction that turns O-phospho-L-serine into L-cysteine sulfonic acid. CDO1, L-cysteine dioxygenase; CSAD, L-cysteine sulfinic acid decarboxylase


Usage

Characterization

pSAA-PlacI-csad

Fig. 2. Confirmation of csad fragment by PCR. M: Marker; Lane 1: csad (1368 bp)

Fig. 3. Confirmation of pSAA fragment by digestion. M: Marker; Lane 1: pSAA (2531 bp)


References
Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 476
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 261