Difference between revisions of "Part:BBa K3714002"
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<center>[[File:K3714002-1.png]]</center> | <center>[[File:K3714002-1.png]]</center> | ||
− | <center><b>Figure. | + | <center><b>Figure.2 | Verification of cleavage property of aptazyme. </b> T7 transcription products were loaded on a denatured PAGE to determine its cleave fraction.</center> |
− | <center>[[File:K3714002 | + | <center>[[File:K3714002.png]]</center> |
<center><b>Figure.3 | Verification of lateral flow assay. </b> T7 transcription product with a target concentration gradient were used as input of LFA.</center> | <center><b>Figure.3 | Verification of lateral flow assay. </b> T7 transcription product with a target concentration gradient were used as input of LFA.</center> | ||
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+ | <center>[[File:K3714002-2.png]]</center> | ||
+ | <center><b>Figure.4 | Performance of lateral flow assay. </b> The strips in Figure.3 were analyzed by ImageJ, and we used a two-phased linear regression to fit the results.</center> | ||
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Revision as of 02:56, 22 October 2021
Biosensor gard-337 for lateral flow assay
The template of aptazyme gard-337 with two probes (binding BBa_K3714007 and BBa_K3714008) on both terminal for lateral flow assay (LFA). It could be used as a biosensor of gardiquimod to verify our design on a colloidal gold based detection platform (Figure.1).
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]