Difference between revisions of "Part:BBa K3777026"

Latest revision as of 02:56, 22 October 2021

KB2-mphR-ermC-T7(mphO)-3WJdB-PmphR-sgRNA

Basic biosensor device for erythromycin detection.

Usage and Biology
Compared to our another mphR-ermC-T7(mphO)-3WJdB(BBa_K3777021),it adds a binding site which can be regonized by dcas9 proteins and sgRNA.(Fig 1)

When Ery was absent, mphR would bind to the inducible promoter（PI）and prevent RNA polymerase from initiating transcription, thus repressing the expression of reporter gene. If tet was present, TetR would no longer able to bind to the promoter, resulting in the expression of reporter gene. But KB2 will bind to 3WJDB leading to lower fluorescent .

Reference：Alam Khalid K,Tawiah Kwaku D,Lichte Matthew F,Porciani David,Burke Donald H. A Fluorescent Split Aptamer for Visualizing RNA-RNA Assembly In Vivo.[J]. ACS synthetic biology,2017,6(9)
Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
INCOMPATIBLE WITH RFC[12]
Illegal NheI site found at 260
Illegal NheI site found at 283
Illegal NheI site found at 1599
Illegal NheI site found at 2015
21
INCOMPATIBLE WITH RFC[21]
Illegal BamHI site found at 1493
Illegal XhoI site found at 121
Illegal XhoI site found at 1144
Illegal XhoI site found at 2139
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
COMPATIBLE WITH RFC[1000]

@@ Line 9: / Line 9: @@
 <br>Compared to our another mphR-ermC-T7(mphO)-3WJdB(BBa_K3777021),it adds a binding site which can be regonized by dcas9 proteins and sgRNA.(Fig 1)
 <br>
-<br>When Ery was absent, mphR would bind to the inducible promoter（PI）and prevent RNA polymerase from initiating transcription, thus repressing the expression of reporter gene. If tet was present, TetR would no longer able to bind to the promoter, resulting in the expression of reporter gene. But BS2 will be regonized and cut by dca9 proteins and sgRNA leading to lower fluorescence expression.
+<br>When Ery was absent, mphR would bind to the inducible promoter（PI）and prevent RNA polymerase from initiating transcription, thus repressing the expression of reporter gene. If tet was present, TetR would no longer able to bind to the promoter, resulting in the expression of reporter gene. But KB2 will bind to 3WJDB leading to lower fluorescent .
 https://static.igem.org/mediawiki/parts/thumb/e/ee/KB2-mphR-ermC-T7%28mphO%29-3WJdB-PmphR-sgRNA.PNG/800px-KB2-mphR-ermC-T7%28mphO%29-3WJdB-PmphR-sgRNA.PNG
+<br>Reference：Alam Khalid K,Tawiah Kwaku D,Lichte Matthew F,Porciani David,Burke Donald H. A Fluorescent Split Aptamer for Visualizing RNA-RNA Assembly In Vivo.[J]. ACS synthetic biology,2017,6(9)
+<br>
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 <span class='h3bb'>Sequence and Features</span>