Difference between revisions of "Part:BBa K3838321"
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1. The DNA level | 1. The DNA level | ||
The plasmid PET-Phok was selected for verification, and the transformed plasmid DH5a (phoK) was verified by plasmid running gel and PCR. As can be seen from Figure 1, the sample band was over 5000bp, basically in line with the target band of 7863bp, which proved that our plasmid transformation was successful. | The plasmid PET-Phok was selected for verification, and the transformed plasmid DH5a (phoK) was verified by plasmid running gel and PCR. As can be seen from Figure 1, the sample band was over 5000bp, basically in line with the target band of 7863bp, which proved that our plasmid transformation was successful. | ||
| − | [[File:T--SZU-China--resultfigure32A.png| | + | |
| + | [[File:T--SZU-China--resultfigure32A.png|400px|Figure1|centre]] | ||
We also carried out enzyme digestion verification on the plasmid. Marker was 10000bp. Sample 1 was the target plasmid, and sample 2 was the sample with endonuclease Smal and EcoRl added for enzyme digestion. There was also a smaller band between 5000bp and 6000bp, which was consistent with the target band 5239bp obtained by enzyme digestion, which further indicated that our plasmid transformation was successful.(Figure 2) | We also carried out enzyme digestion verification on the plasmid. Marker was 10000bp. Sample 1 was the target plasmid, and sample 2 was the sample with endonuclease Smal and EcoRl added for enzyme digestion. There was also a smaller band between 5000bp and 6000bp, which was consistent with the target band 5239bp obtained by enzyme digestion, which further indicated that our plasmid transformation was successful.(Figure 2) | ||
| − | [[File:T--SZU-China--resultfigure32B.png| | + | [[File:T--SZU-China--resultfigure32B.png|400px|Figure2|centre]] |
Latest revision as of 02:56, 22 October 2021
phoKiller
Allowing bacteria to sense phosphate concentrations in the environment to act on the kill switch mechanism.
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Data:SZU-China 2021 TEAM
1. The DNA level The plasmid PET-Phok was selected for verification, and the transformed plasmid DH5a (phoK) was verified by plasmid running gel and PCR. As can be seen from Figure 1, the sample band was over 5000bp, basically in line with the target band of 7863bp, which proved that our plasmid transformation was successful.
We also carried out enzyme digestion verification on the plasmid. Marker was 10000bp. Sample 1 was the target plasmid, and sample 2 was the sample with endonuclease Smal and EcoRl added for enzyme digestion. There was also a smaller band between 5000bp and 6000bp, which was consistent with the target band 5239bp obtained by enzyme digestion, which further indicated that our plasmid transformation was successful.(Figure 2)
