Difference between revisions of "Part:BBa K3738005:Design"

(→‎Design Notes)
(→‎References)
 
(5 intermediate revisions by the same user not shown)
Line 7: Line 7:
  
 
===Design Notes===
 
===Design Notes===
−
The design of this part was based off of part BBa_K2683012, which is an MS2 capsid protein fused to a polyarginine tag cell-penetrating peptide on the N-terminus.  This part has been modified from the original so as to have the polyarginine tag replaced with a histidine tag, to allow for purification as a monomer for capsid assembly in vitro. This part has been codon-optimized for use in E.coli.
+
This part is an improved version of the part BBa_K2683012, and has been modified to include an N-terminal histidine tag and has had the polyarginine tag removed.
  
 
This part has been codon optimized for use in E.coli.
 
This part has been codon optimized for use in E.coli.
Line 13: Line 13:
 
===Source===
 
===Source===
  
−
BBa_K2683012
+
This part comes from the MS2 bacteriophage.
  
 
===References===
 
===References===
 +
[1] Glasgow, J., Capehart, S., Francis, M., and Tullman-Ercek, D. (2012) Osmolyte-Mediated Encapsulation of Proteins inside MS2 viral capsids. ACSNAno. 6, 8658-8664
 +
 +
[2] Vives, E., Schmidt, J., And Pelegrin, A. (2008) Cell penetrating and cell-targeting peptides in drug delivery. Biochimica et biophysica Acta. 1786, 126-138
 +
 +
https://www.uniprot.org/uniprot/P03612

Latest revision as of 02:49, 22 October 2021


MS2 Bacteriophage Capsid Protein with N-Terminal Histidine Tag


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

This part is an improved version of the part BBa_K2683012, and has been modified to include an N-terminal histidine tag and has had the polyarginine tag removed.

This part has been codon optimized for use in E.coli.

Source

This part comes from the MS2 bacteriophage.

References

[1] Glasgow, J., Capehart, S., Francis, M., and Tullman-Ercek, D. (2012) Osmolyte-Mediated Encapsulation of Proteins inside MS2 viral capsids. ACSNAno. 6, 8658-8664

[2] Vives, E., Schmidt, J., And Pelegrin, A. (2008) Cell penetrating and cell-targeting peptides in drug delivery. Biochimica et biophysica Acta. 1786, 126-138

https://www.uniprot.org/uniprot/P03612