Difference between revisions of "Part:BBa K3738005:Design"

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===Design Notes===
 
===Design Notes===
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The design of this part was based of part BBa_K2683012, which is an MS2 capsid protein fused to a polyarginine tag cell-penetrating peptide on the N-terminus.  This part has been modified from the original so as to have the polyarginine tag replaced with a histidine tag, to allow for purification as a monomer for capsid assembly in vitro. This part has been codon-optimized for use in E.coli.
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This part is an improved version of the part BBa_K2683012, and has been modified to include an N-terminal histidine tag and has had the polyarginine tag removed.
  
 
This part has been codon optimized for use in E.coli.
 
This part has been codon optimized for use in E.coli.
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===Source===
 
===Source===
  
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BBa_K2683012
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This part comes from the MS2 bacteriophage.
  
 
===References===
 
===References===
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[1] Glasgow, J., Capehart, S., Francis, M., and Tullman-Ercek, D. (2012) Osmolyte-Mediated Encapsulation of Proteins inside MS2 viral capsids. ACSNAno. 6, 8658-8664
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[2] Vives, E., Schmidt, J., And Pelegrin, A. (2008) Cell penetrating and cell-targeting peptides in drug delivery. Biochimica et biophysica Acta. 1786, 126-138
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https://www.uniprot.org/uniprot/P03612

Latest revision as of 02:49, 22 October 2021


MS2 Bacteriophage Capsid Protein with N-Terminal Histidine Tag


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

This part is an improved version of the part BBa_K2683012, and has been modified to include an N-terminal histidine tag and has had the polyarginine tag removed.

This part has been codon optimized for use in E.coli.

Source

This part comes from the MS2 bacteriophage.

References

[1] Glasgow, J., Capehart, S., Francis, M., and Tullman-Ercek, D. (2012) Osmolyte-Mediated Encapsulation of Proteins inside MS2 viral capsids. ACSNAno. 6, 8658-8664

[2] Vives, E., Schmidt, J., And Pelegrin, A. (2008) Cell penetrating and cell-targeting peptides in drug delivery. Biochimica et biophysica Acta. 1786, 126-138

https://www.uniprot.org/uniprot/P03612