Difference between revisions of "Part:BBa K4032003"

Latest revision as of 02:40, 22 October 2021

amylase-RFP

This part contains the gene of the amylase-RFP fusion protein.

For more information on the amylase gene malS, see NCBI: NC_000913.3

Usage and Biology

This enzyme hydrolyzes of (1-4)-α-D-glycosidic linkages in polysaccharides containing three or more (1-4)-α-linked D-glucose units. For more information, see NCBI: NC_000913.3

In the case of this part, red fluorescence of RFP is observed with a fluorescence microscope, but isn’t observed under the natural light source.

This part expresses the fusion protein of amylase from BBa_K523006 and RFP from BBa_J04450. We removed the stop codon of the amylase gene and the start codon of the RFP gene and connected the two ends.

The RBS is added to this part, forming BBa_K4032006.

The lac promoter, the RBS and the double terminator are added to this part, forming BBa_K4032104.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
INCOMPATIBLE WITH RFC[12]
Illegal NheI site found at 1200
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
INCOMPATIBLE WITH RFC[25]
Illegal AgeI site found at 336
Illegal AgeI site found at 1501
Illegal AgeI site found at 2580
Illegal AgeI site found at 2692
1000
COMPATIBLE WITH RFC[1000]

@@ Line 6: / Line 6: @@
 This part contains the gene of the amylase-RFP fusion protein.
-See [https://www.ncbi.nlm.nih.gov/gene/948088 NCBI: NC_000913.3]for information on the amylase gene ''malS''.
+For more information on the amylase gene ''malS'', see [https://www.ncbi.nlm.nih.gov/gene/948088 NCBI: NC_000913.3]
 ===Usage and Biology===
 This enzyme hydrolyzes of (1-4)-α-D-glycosidic linkages in polysaccharides containing three or more (1-4)-α-linked D-glucose units.
-See [https://www.ncbi.nlm.nih.gov/gene/948088 NCBI: NC_000913.3]for details.
+For more information, see [https://www.ncbi.nlm.nih.gov/gene/948088 NCBI: NC_000913.3]
-In the case of this part,  red fluorescence of RFP is observed with a fluorescence microscope, but isn’t observed under the natural light source.
+In the case of this part, red fluorescence of RFP is observed with a fluorescence microscope, but isn’t observed under the natural light source.
-This part expresses the fusion protein of amylase (from <partinfo>BBa_K523006</partinfo>) and RFP (from <partinfo>BBa_J04450</partinfo>). We removed the stop codon of the amylase gene and the start codon of the RFP gene and connected the two ends.
+This part expresses the fusion protein of amylase from <partinfo>BBa_K523006</partinfo> and RFP from <partinfo>BBa_J04450</partinfo>. We removed the stop codon of the amylase gene and the start codon of the RFP gene and connected the two ends.