Difference between revisions of "Part:BBa K3738019"

Latest revision as of 02:25, 22 October 2021

MlrA for E.coli

MlrA, or microcystinase, cleaves the peptide backbone of MC-LR cyanotoxins produced by microcystis aeruginosa cyanobacteria. This crucial step results in the peptide losing the cyclic structure, and therefore the majority of its toxicity. Further degradation continues in an enzymatic pathway by interactions with other peptidolytic enzymes from the microcystinase gene cluster. This design for this part was inspired by the Aalto-Helsinki 2016 iGEM team (Part BBa_K1907001), but was originally codon-optimized for yeast. We have modified this part by codon-optimizing it for use in E.coli. Additionally, team Aalto-Helsinki's mlrA came from Sphingomonas sp. ACM-3962, whereas this part originates from Sphingomonas sp. USTB-05.

Sequence and Features

Assembly Compatibility:

10
INCOMPATIBLE WITH RFC[10]
Illegal EcoRI site found at 7
Illegal EcoRI site found at 868
12
INCOMPATIBLE WITH RFC[12]
Illegal EcoRI site found at 7
Illegal EcoRI site found at 868
21
INCOMPATIBLE WITH RFC[21]
Illegal EcoRI site found at 7
Illegal EcoRI site found at 868
23
INCOMPATIBLE WITH RFC[23]
Illegal EcoRI site found at 7
Illegal EcoRI site found at 868
25
INCOMPATIBLE WITH RFC[25]
Illegal EcoRI site found at 7
Illegal EcoRI site found at 868
1000
COMPATIBLE WITH RFC[1000]

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 <partinfo>BBa_K3738019 short</partinfo>
-MlrA, or microcystinase, cleaves the peptide backbone of MC-LR cyanotoxins produced by microcystis aeruginosa cyanobacteria. This crucial step results in the peptide losing the cyclic structure, and therefore the majority of its toxicity. Further degradation continues in an enzymatic pathway by interactions with other peptidolytic enzymes from the microcystinase gene cluster. This part originally comes from the Aalto-Helsinki 2016 iGEM team (Part BBa_K1907001), but was originally codon-optimized for yeast. We have modified this part by codon-optimizing it for use in E.coli.
+MlrA, or microcystinase, cleaves the peptide backbone of MC-LR cyanotoxins produced by microcystis aeruginosa cyanobacteria. This crucial step results in the peptide losing the cyclic structure, and therefore the majority of its toxicity. Further degradation continues in an enzymatic pathway by interactions with other peptidolytic enzymes from the microcystinase gene cluster. This design for this part was inspired by the Aalto-Helsinki 2016 iGEM team (Part BBa_K1907001), but was originally codon-optimized for yeast. We have modified this part by codon-optimizing it for use in E.coli. Additionally, team Aalto-Helsinki's mlrA came from Sphingomonas sp. ACM-3962, whereas this part originates from Sphingomonas sp. USTB-05.
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