Difference between revisions of "Part:BBa K3739002"

 
 
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<partinfo>BBa_K3739002 short</partinfo>
 
<partinfo>BBa_K3739002 short</partinfo>
  
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CBM can anchor onto cellulose and GFP can show whether CenA anchor or not. Becteria that is used to store our plasmid
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CBM can anchor onto cellulose and GFP can show whether CenA anchor or not.  
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===Usage and Biology===
  
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=====CBM=====
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Cellulose enzymes have two domains, and the one that helps bind to cellulose is called cellulose binding module (CBM), and therefore it helps our fusion protein bind to cellulose-rich cell wall. Here we choose the CBM of CenA from Cellulomonas fimi, which has been successfully expressed in Escherichia coli.
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=====GFP=====
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Protein tags are peptides genetically fused to the proteins of interest. There are many different kinds of tags developed for different purposes. For labeling experiments, normally the proteins of interest are tagged directly by fluorescent tags. Green fluorescent protein (GFP) make it easier to study protein localization, interactions and dynamics when fusing with other peptides and proteins.
  
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===Usage and Biology===
 
  
 
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Latest revision as of 02:13, 22 October 2021


his-GFP-CBM

CBM can anchor onto cellulose and GFP can show whether CenA anchor or not.

Usage and Biology

CBM

Cellulose enzymes have two domains, and the one that helps bind to cellulose is called cellulose binding module (CBM), and therefore it helps our fusion protein bind to cellulose-rich cell wall. Here we choose the CBM of CenA from Cellulomonas fimi, which has been successfully expressed in Escherichia coli.

GFP

Protein tags are peptides genetically fused to the proteins of interest. There are many different kinds of tags developed for different purposes. For labeling experiments, normally the proteins of interest are tagged directly by fluorescent tags. Green fluorescent protein (GFP) make it easier to study protein localization, interactions and dynamics when fusing with other peptides and proteins.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 1042
    Illegal AgeI site found at 1132
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 73