Difference between revisions of "Part:BBa K4044008"

Latest revision as of 02:02, 22 October 2021

BphP1_QPAS1-Gal4 optogenetic system

The BphP1 monomer competes for binding to the QPAS1 domain, which leads to the rupture of the Gal4-QPAS1 dimer and its dissociation from DNA. Under the influence of red light with a wavelength of 640 nm, active BphP1 passes into an inactivated dimerized state. In this case, Gal4-QPAS1 reverts to the dimer state, binds to DNA and suppresses transcription of the reporter gene. Bacteriophytochrome BphP1 in the inactive state is an intact dimer. BphP1 changes from an inactive dimerized state (Pr) to an active monomeric state (Pfr) under the influence of radiation at 760 nm, which opens the binding site for its contacting compound, Q-PAS1. The Gal4 transcription factor is attached to it via a peptide bond. Gal4-QPAS1 dimer bounds to DNA via two sites of trinucleotides in the promoter region for each Gal4 HTH-domain, inhibiting transcription of the reporter gene. Under the influence of far red light with a wavelength of 760 nm, the expression of target genes is activated. There are several states of the ontogenetic system. Under the influence of far red light, BphP1 part of the chimeric protein BphP1 becomes active and binds to QPAS1-Gal4. This leads to the expression of target gene. Under the influence of red light, BphP1 is inactivated, QPAS1-Gal4 remains in the dimerized state. This leads to inhibition of transcription.

Sequence and Features

Assembly Compatibility:

10
INCOMPATIBLE WITH RFC[10]
Illegal PstI site found at 815
Illegal PstI site found at 1112
Illegal PstI site found at 1559
12
INCOMPATIBLE WITH RFC[12]
Illegal NheI site found at 7
Illegal NheI site found at 30
Illegal PstI site found at 815
Illegal PstI site found at 1112
Illegal PstI site found at 1559
21
INCOMPATIBLE WITH RFC[21]
Illegal BamHI site found at 301
Illegal BamHI site found at 1876
Illegal BamHI site found at 2969
23
INCOMPATIBLE WITH RFC[23]
Illegal PstI site found at 815
Illegal PstI site found at 1112
Illegal PstI site found at 1559
25
INCOMPATIBLE WITH RFC[25]
Illegal PstI site found at 815
Illegal PstI site found at 1112
Illegal PstI site found at 1559
Illegal NgoMIV site found at 457
Illegal NgoMIV site found at 1208
Illegal NgoMIV site found at 1705
1000
COMPATIBLE WITH RFC[1000]

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 <partinfo>BBa_K4044008 short</partinfo>
-The BphP1 monomer competes for binding to the QPAS 1 domain, which leads to the rupture of the Gal4-QPAS 1 dimer and its dissociation from DNA. Under the influence of red light with a wavelength of 640 nm, active BphP1 passes into an inactivated dimerized state. In this case, Gal4-QPAS 1 reverts to the dimer state, binds to DNA and suppresses transcription of the reporter gene.
+The BphP1 monomer competes for binding to the QPAS1 domain, which leads to the rupture of the Gal4-QPAS1 dimer and its dissociation from DNA. Under the influence of red light with a wavelength of 640 nm, active BphP1 passes into an inactivated dimerized state. In this case, Gal4-QPAS1 reverts to the dimer state, binds to DNA and suppresses transcription of the reporter gene.
-Bacterio phyto chromium Bp hP 1 in the inactive state is an intact dimer. Bp hP 1 changes from an inactive dimerized state (Pr) to an active monomeric state (Zac) under the influence of radiation at 760 nm, which opens the binding site for its contacting compound Q-PAS1. The Gal4 transcription factor is attached to it via a peptide bond. Gal4-QPAS1 dimer bounds to DNA via two sites of  trinucleotides in the promoter region for each Gal4 HTH domain, inhibiting transcription of the reporter gene. Under the influence of far red light with a wavelength of 760 nm, the expression of target genes is activated
+Bacteriophytochrome BphP1 in the inactive state is an intact dimer. BphP1 changes from an inactive dimerized state (Pr) to an active monomeric state (Pfr) under the influence of radiation at 760 nm, which opens the binding site for its contacting compound, Q-PAS1. The Gal4 transcription factor is attached to it via a peptide bond. Gal4-QPAS1 dimer bounds to DNA via two sites of trinucleotides in the promoter region for each Gal4 HTH-domain, inhibiting transcription of the reporter gene. Under the influence of far red light with a wavelength of 760 nm, the expression of target genes is activated.
 There are several states of the ontogenetic system. Under the influence of far red light, BphP1 part of the chimeric protein BphP1 becomes active and binds to QPAS1-Gal4. This leads to the expression of target gene. Under the influence of red light, BphP1 is inactivated, QPAS1-Gal4 remains in the dimerized state. This leads to inhibition of transcription.