Difference between revisions of "Part:BBa K3739003"
(→Characterization) |
(→Characterization) |
||
| Line 16: | Line 16: | ||
We construct his-GFP CDS with J23100 promotor and B0030 RBS, the transformed cells are selected by colony PCR. The experiment result is showed in Fig. 1. <br/> | We construct his-GFP CDS with J23100 promotor and B0030 RBS, the transformed cells are selected by colony PCR. The experiment result is showed in Fig. 1. <br/> | ||
<!-- --> | <!-- --> | ||
| − | [[File:T--XMU-China--his-GFP.png|200px|alt text]]<br/> | + | <center>[[File:T--XMU-China--his-GFP.png|200px|alt text]]</center><br/> |
Fig. 1. DNA gel electrophoresis of colony PCR product of J23100-B0030-his-GFP-pSB1C3. | Fig. 1. DNA gel electrophoresis of colony PCR product of J23100-B0030-his-GFP-pSB1C3. | ||
<span class='h3bb'>Sequence and Features</span> | <span class='h3bb'>Sequence and Features</span> | ||
Revision as of 01:49, 22 October 2021
his-GFP
his-tag is used to be detected by anti-his antibody and purify the his-linking fusion, GFP can emit green fluorescence.
Biology
GFP Protein tags are peptides genetically fused to the proteins of interest. There are many different kinds of tags developed for for different purposes. For labeling experiments, normally the proteins of interest are tagged directly by fluorescent tags. Green fluorescent protein (GFP) make it easier to study protein localization, interactions and dynamics when fusing with other peptides and proteins.
Usage
Used to construct the expression system and obtained the composite part BBa_K3739043.
Characterization
1.Colony PCR
We construct his-GFP CDS with J23100 promotor and B0030 RBS, the transformed cells are selected by colony PCR. The experiment result is showed in Fig. 1.
Fig. 1. DNA gel electrophoresis of colony PCR product of J23100-B0030-his-GFP-pSB1C3. Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal SapI.rc site found at 73