Difference between revisions of "Part:BBa K3771013"
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− | <br><i>P<sub>lacI</sub></i> | + | <br><i>P<sub>lacI</sub>-ofp</i> is part of the IFN-γ sensing system and is the initial composite part used to construct plasmids used in the IFN-γ sensing system experiments.<br> |
<br><b style="font-size:1.3rem">Biology</b> | <br><b style="font-size:1.3rem">Biology</b> | ||
<br> | <br> | ||
− | <br>Without the presence of the LacI repressor, the | + | <br>Without the presence of the LacI repressor, the <i>lacI</i> promoter facilitates the constitutive expression of OFP.<br> |
<br><b style="font-size:1.3rem">Characterization</b> | <br><b style="font-size:1.3rem">Characterization</b> | ||
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<img src="https://2021.igem.org/wiki/images/0/01/T--NCKU_Tainan--composite_pLacI_OFP.jpg" style="width:35%;"> | <img src="https://2021.igem.org/wiki/images/0/01/T--NCKU_Tainan--composite_pLacI_OFP.jpg" style="width:35%;"> | ||
</div> | </div> | ||
− | <p align="center">Fig. 1. Double digestion of <i>P<sub>lacI</sub></i> | + | <p align="center">Fig. 1. Double digestion of <i>P<sub>lacI</sub>-ofp</i></p> |
Latest revision as of 01:33, 22 October 2021
PlacI-OFP
Description
PlacI-ofp is part of the IFN-γ sensing system and is the initial composite part used to construct plasmids used in the IFN-γ sensing system experiments.
Biology
Without the presence of the LacI repressor, the lacI promoter facilitates the constitutive expression of OFP.
Characterization
Double digestion results are shown in Fig. 1.
![](https://2021.igem.org/wiki/images/0/01/T--NCKU_Tainan--composite_pLacI_OFP.jpg)
Fig. 1. Double digestion of PlacI-ofp
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]