Difference between revisions of "Part:BBa K3771013"
 
(7 intermediate revisions by the same user not shown)
Line 2: Line 2:
 
__NOTOC__
 
__NOTOC__
 
<partinfo>BBa_K3771013 short</partinfo>
 
<partinfo>BBa_K3771013 short</partinfo>
 +
 +
<html>
  
 
<br><b style="font-size:1.3rem">Description</b>
 
<br><b style="font-size:1.3rem">Description</b>
 
<br>
 
<br>
  
−
<br><i>P<sub>lacI</sub></i>-OFP is part of the IFN-γ sensing system and is the initial composite part used to construct plasmids used in the IFN-γ sensing system experiments.<br>
+
<br><i>P<sub>lacI</sub>-ofp</i> is part of the IFN-γ sensing system and is the initial composite part used to construct plasmids used in the IFN-γ sensing system experiments.<br>
  
 
<br><b style="font-size:1.3rem">Biology</b>
 
<br><b style="font-size:1.3rem">Biology</b>
 
<br>
 
<br>
  
−
<br>Without the presence of the LacI repressor, the LacI promoter facilitates the constitutive expression of OFP.<br>
+
  <br>Without the presence of the LacI repressor, the <i>lacI</i> promoter facilitates the constitutive expression of OFP.<br>
  
 
<br><b style="font-size:1.3rem">Characterization</b>
 
<br><b style="font-size:1.3rem">Characterization</b>
 
<br>
 
<br>
  
 +
<br>Double digestion results are shown in Fig. 1.<br>
 +
 
 
<div style="width=100%; display:flex; align-items: center; justify-content: center;">
 
<div style="width=100%; display:flex; align-items: center; justify-content: center;">
 
<img src="https://2021.igem.org/wiki/images/0/01/T--NCKU_Tainan--composite_pLacI_OFP.jpg" style="width:35%;">
 
<img src="https://2021.igem.org/wiki/images/0/01/T--NCKU_Tainan--composite_pLacI_OFP.jpg" style="width:35%;">
 
</div>
 
</div>
−
<p align="center">Double digestion results are shown in Fig. 2.</p>
+
  <p align="center">Fig. 1. Double digestion of <i>P<sub>lacI</sub>-ofp</i></p>
−
 
+
  
  
 +
</html>
  
 
<!-- Add more about the biology of this part here
 
<!-- Add more about the biology of this part here

Latest revision as of 01:33, 22 October 2021


PlacI-OFP


Description

PlacI-ofp is part of the IFN-γ sensing system and is the initial composite part used to construct plasmids used in the IFN-γ sensing system experiments.

Biology

Without the presence of the LacI repressor, the lacI promoter facilitates the constitutive expression of OFP.

Characterization

Double digestion results are shown in Fig. 1.

Fig. 1. Double digestion of PlacI-ofp

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]