Difference between revisions of "Part:BBa K3795000:Experience"
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===Applications of DME-C-BBa_K3795000=== | ===Applications of DME-C-BBa_K3795000=== | ||
| − | In order to evaluate if DME-C could recognize anti-Dengue antibodies, western blot analysis were performed with | + | In order to evaluate if DME-C could recognize anti-Dengue antibodies, western blot analysis were performed with anti-Dengue envelope protein. Fortunately, both DME-C and DME-BR were capable of recognizing anti-Dengue E antibodies, showing that Ammit fullfiled at least its main function. However, the preliminary anti-Zika assays showed that the protein also recognized anti-Zika antibodies (Figures 1 and 2). |
https://static.igem.org/mediawiki/parts/9/9f/T--Rio_UFRJ_Brazil--Anti-E-peq-denv.png <br> | https://static.igem.org/mediawiki/parts/9/9f/T--Rio_UFRJ_Brazil--Anti-E-peq-denv.png <br> | ||
| − | <br> Anti-Dengue Envelope (Invitrogen) protein Western blot analysis. We used Anti - Mouse IRDye® 800CW (LI-COR), at 1:10,000, as the secondary antibody. M: Protein marker (Spectra™ Multicolor Low Range Protein, Thermo). 1 - 3: Different dengue virus lysate containing 30 μg of protein used as a positive control. 4: 17 μg of DME-C. 5: 17 μg of DME-BR.<br> | + | <br> Figure 1: Anti-Dengue Envelope (Invitrogen) protein Western blot analysis. We used Anti - Mouse IRDye® 800CW (LI-COR), at 1:10,000, as the secondary antibody. M: Protein marker (Spectra™ Multicolor Low Range Protein, Thermo). 1 - 3: Different dengue virus lysate containing 30 μg of protein used as a positive control. 4: 17 μg of DME-C. 5: 17 μg of DME-BR.<br> |
<br> https://static.igem.org/mediawiki/parts/2/28/T--Rio_UFRJ_Brazil--Anti-Ns1-peq-Zika.png <br> | <br> https://static.igem.org/mediawiki/parts/2/28/T--Rio_UFRJ_Brazil--Anti-Ns1-peq-Zika.png <br> | ||
| − | (1)e(2)MW Molecular Weight Marker, Unstained Protein Molecular Weight Marker; (3) 2.0μg DMEC sample; (4) 1.0μg DMEC sample; (5) Sample of 2.0μg DMEC; (6) DMEBR 2.0μg sample; (7) DMEBR 1.0μg sample; (8) DMEBR 0.5μg sample; <br> | + | Figure 2: Anti-Zika NS1 Western Blot analysis. (1)e(2)MW Molecular Weight Marker, Unstained Protein Molecular Weight Marker; (3) 2.0μg DMEC sample; (4) 1.0μg DMEC sample; (5) Sample of 2.0μg DMEC; (6) DMEBR 2.0μg sample; (7) DMEBR 1.0μg sample; (8) DMEBR 0.5μg sample; <br> |
===User Reviews=== | ===User Reviews=== | ||
Latest revision as of 01:29, 22 October 2021
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Applications of DME-C-BBa_K3795000
In order to evaluate if DME-C could recognize anti-Dengue antibodies, western blot analysis were performed with anti-Dengue envelope protein. Fortunately, both DME-C and DME-BR were capable of recognizing anti-Dengue E antibodies, showing that Ammit fullfiled at least its main function. However, the preliminary anti-Zika assays showed that the protein also recognized anti-Zika antibodies (Figures 1 and 2).
Figure 1: Anti-Dengue Envelope (Invitrogen) protein Western blot analysis. We used Anti - Mouse IRDye® 800CW (LI-COR), at 1:10,000, as the secondary antibody. M: Protein marker (Spectra™ Multicolor Low Range Protein, Thermo). 1 - 3: Different dengue virus lysate containing 30 μg of protein used as a positive control. 4: 17 μg of DME-C. 5: 17 μg of DME-BR.
Figure 2: Anti-Zika NS1 Western Blot analysis. (1)e(2)MW Molecular Weight Marker, Unstained Protein Molecular Weight Marker; (3) 2.0μg DMEC sample; (4) 1.0μg DMEC sample; (5) Sample of 2.0μg DMEC; (6) DMEBR 2.0μg sample; (7) DMEBR 1.0μg sample; (8) DMEBR 0.5μg sample;
User Reviews
UNIQ2251d20b617cd9c2-partinfo-00000000-QINU UNIQ2251d20b617cd9c2-partinfo-00000001-QINU