Difference between revisions of "Part:BBa K3795000:Experience"

Latest revision as of 01:29, 22 October 2021

This experience page is provided so that any user may enter their experience using this part.
Please enter how you used this part and how it worked out.

Applications of DME-C-BBa_K3795000

In order to evaluate if DME-C could recognize anti-Dengue antibodies, western blot analysis were performed with anti-Dengue envelope protein. Fortunately, both DME-C and DME-BR were capable of recognizing anti-Dengue E antibodies, showing that Ammit fullfiled at least its main function. However, the preliminary anti-Zika assays showed that the protein also recognized anti-Zika antibodies (Figures 1 and 2).

Figure 1: Anti-Dengue Envelope (Invitrogen) protein Western blot analysis. We used Anti - Mouse IRDye® 800CW (LI-COR), at 1:10,000, as the secondary antibody. M: Protein marker (Spectra™ Multicolor Low Range Protein, Thermo). 1 - 3: Different dengue virus lysate containing 30 μg of protein used as a positive control. 4: 17 μg of DME-C. 5: 17 μg of DME-BR.

Figure 2: Anti-Zika NS1 Western Blot analysis. (1)e(2)MW Molecular Weight Marker, Unstained Protein Molecular Weight Marker; (3) 2.0μg DMEC sample; (4) 1.0μg DMEC sample; (5) Sample of 2.0μg DMEC; (6) DMEBR 2.0μg sample; (7) DMEBR 1.0μg sample; (8) DMEBR 0.5μg sample;

User Reviews

UNIQb88eff884f7f3e2a-partinfo-00000000-QINU UNIQb88eff884f7f3e2a-partinfo-00000001-QINU

@@ Line 5: / Line 5: @@
 ===Applications of DME-C-BBa_K3795000===
-In order to evaluate if DME-C could recognize anti-Dengue antibodies, western blot analysis were performed with two different commercial antibodies: anti-Dengue NS1, and anti-Dengue envelope protein. Fortunately, both DME-C and DME-BR were capable of recognizing anti-Dengue E antibodies, showing that Ammit fullfiled at least its main function. However, the preliminary anti-Zika assays showed that the protein also recognized anti-Zika antibodies.
+In order to evaluate if DME-C could recognize anti-Dengue antibodies, western blot analysis were performed with anti-Dengue envelope protein. Fortunately, both DME-C and DME-BR were capable of recognizing anti-Dengue E antibodies, showing that Ammit fullfiled at least its main function. However, the preliminary anti-Zika assays showed that the protein also recognized anti-Zika antibodies (Figures 1 and 2).
 https://static.igem.org/mediawiki/parts/9/9f/T--Rio_UFRJ_Brazil--Anti-E-peq-denv.png <br>
- Anti-Dengue Envelope (Invitrogen) protein Western blot analysis. We used   Anti - Mouse IRDye® 800CW (LI-COR), at 1:10,000, as the secondary antibody. M: Protein marker (Spectra™ Multicolor Low Range Protein, Thermo). 1 - 3: Different dengue virus lysate containing 30 μg of protein used as a positive control. 4: 17 μg of DME-C. 5: 17 μg of DME-BR.
+<br> Figure 1: Anti-Dengue Envelope (Invitrogen) protein Western blot analysis. We used   Anti - Mouse IRDye® 800CW (LI-COR), at 1:10,000, as the secondary antibody. M: Protein marker (Spectra™ Multicolor Low Range Protein, Thermo). 1 - 3: Different dengue virus lysate containing 30 μg of protein used as a positive control. 4: 17 μg of DME-C. 5: 17 μg of DME-BR.<br>
 <br> https://static.igem.org/mediawiki/parts/2/28/T--Rio_UFRJ_Brazil--Anti-Ns1-peq-Zika.png <br>
-(1)e(2)MW Molecular Weight Marker, Unstained Protein Molecular Weight Marker; (3) 2.0μg DMEC sample; (4) 1.0μg DMEC sample; (5) Sample of 2.0μg DMEC; (6) DMEBR 2.0μg sample; (7) DMEBR 1.0μg sample; (8) DMEBR 0.5μg sample;
+Figure 2: Anti-Zika NS1 Western Blot analysis. (1)e(2)MW Molecular Weight Marker, Unstained Protein Molecular Weight Marker; (3) 2.0μg DMEC sample; (4) 1.0μg DMEC sample; (5) Sample of 2.0μg DMEC; (6) DMEBR 2.0μg sample; (7) DMEBR 1.0μg sample; (8) DMEBR 0.5μg sample; <br>
 ===User Reviews===