Difference between revisions of "Part:BBa K3739024"

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===Biology===
 
===Biology===
 
LamB is an anchor protein from Vibrio Species, which can anchor its passenger protein to the cell membrane. It has been widely used in cell-surface display. LCIKR-2, A mutant of a peptide called LCI from ''Bacillus subtilis'', can sticks to polypropylene strongly. LCIKR-2 is fused at C terminal with LamB so that LCIKR-2 can be displayed on the surface of VnDX.
 
LamB is an anchor protein from Vibrio Species, which can anchor its passenger protein to the cell membrane. It has been widely used in cell-surface display. LCIKR-2, A mutant of a peptide called LCI from ''Bacillus subtilis'', can sticks to polypropylene strongly. LCIKR-2 is fused at C terminal with LamB so that LCIKR-2 can be displayed on the surface of VnDX.
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===Usage===
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Here, we use BBa_3739024 to construct the expression system, which may achieve surface display of LCIKR-2 on VnDX to help the VnDX adhere to polypropylene.
  
 
===Characterization===
 
===Characterization===
 
====1. Identification====
 
====1. Identification====
 
After receiving the synthesized DNA, PCR was done to certify that the plasmid was correct, and the experimental results were shown in figure1.
 
After receiving the synthesized DNA, PCR was done to certify that the plasmid was correct, and the experimental results were shown in figure1.
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https://static.igem.org/mediawiki/parts/9/90/T--XMU-China--K3739051X.png
 
::'''Fig. 1.''' Gene circuit and agarose gel electrophoresis. (A)  Gene circuit of ''LamB-LCIKR-2-GFP'' (BBa_K3739024). (B)Target bands of ''LamB-LCIKR-2-GFP'' (black arrow, 2300 bp).
 
::'''Fig. 1.''' Gene circuit and agarose gel electrophoresis. (A)  Gene circuit of ''LamB-LCIKR-2-GFP'' (BBa_K3739024). (B)Target bands of ''LamB-LCIKR-2-GFP'' (black arrow, 2300 bp).
 
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Latest revision as of 01:08, 22 October 2021


LamB-LC1KR2-GFP

We anchor LCIKR-2 protein onto membranes through LamB to stick the engineered bacteria on the polypropylene. We use BBa_K3739051 to construct the expression system and anchor LCIKR-2 on the surface of VnDX.

Biology

LamB is an anchor protein from Vibrio Species, which can anchor its passenger protein to the cell membrane. It has been widely used in cell-surface display. LCIKR-2, A mutant of a peptide called LCI from Bacillus subtilis, can sticks to polypropylene strongly. LCIKR-2 is fused at C terminal with LamB so that LCIKR-2 can be displayed on the surface of VnDX.

Usage

Here, we use BBa_3739024 to construct the expression system, which may achieve surface display of LCIKR-2 on VnDX to help the VnDX adhere to polypropylene.

Characterization

1. Identification

After receiving the synthesized DNA, PCR was done to certify that the plasmid was correct, and the experimental results were shown in figure1. T--XMU-China--K3739051X.png

Fig. 1. Gene circuit and agarose gel electrophoresis. (A) Gene circuit of LamB-LCIKR-2-GFP (BBa_K3739024). (B)Target bands of LamB-LCIKR-2-GFP (black arrow, 2300 bp).

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 528
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 676
    Illegal SapI.rc site found at 1432