Difference between revisions of "Part:BBa K3729003"

Latest revision as of 01:06, 22 October 2021

Strong promoter + strong rbs + BCD 1 gene + double terminator

The composite part consists of a strong promoter (BBa_J23100), a strong ribosome binding site(RBS), our BCD1 gene (BBa_K3729000), and a double terminator (BBa_K0015). This design of construct would lead to overexpression of the DNA sequence, further upregulating the production of butyryl- CoA dehydrogenase. Our composite part is synthesized by IDT.

Sequence and Features

Assembly Compatibility:

10
INCOMPATIBLE WITH RFC[10]
Illegal EcoRI site found at 1
Illegal EcoRI site found at 113
Illegal SpeI site found at 230
Illegal SpeI site found at 1312
12
INCOMPATIBLE WITH RFC[12]
Illegal EcoRI site found at 1
Illegal EcoRI site found at 113
Illegal NheI site found at 13
Illegal NheI site found at 36
Illegal SpeI site found at 230
Illegal SpeI site found at 1312
21
INCOMPATIBLE WITH RFC[21]
Illegal EcoRI site found at 1
Illegal EcoRI site found at 113
Illegal BamHI site found at 684
23
INCOMPATIBLE WITH RFC[23]
Illegal EcoRI site found at 1
Illegal EcoRI site found at 113
Illegal SpeI site found at 230
Illegal SpeI site found at 1312
25
INCOMPATIBLE WITH RFC[25]
Illegal EcoRI site found at 1
Illegal EcoRI site found at 113
Illegal SpeI site found at 230
Illegal SpeI site found at 1312
1000
COMPATIBLE WITH RFC[1000]

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 The composite part consists of a strong promoter (BBa_J23100), a strong ribosome binding site(RBS), our BCD1 gene (BBa_K3729000), and a double terminator (BBa_K0015). This design of construct would lead to overexpression of the DNA sequence, further upregulating the production of butyryl- CoA dehydrogenase. Our composite part is synthesized by IDT.
-<!-- Add more about the biology of this part here
+<img src="https://static.igem.org/mediawiki/parts/d/d0/T--KCIS_New_Taipei--BCD1_complex.png" />
-===Usage and Biology===
 <!-- -->