Difference between revisions of "Part:BBa K4046620"
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[[File:T--Duke--hUBCcLuc.png|800 px]] <br>
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[[File:T--Duke--hUBC Curve.png|800 px]] <br>
 
<b><font size="+1">Figure 1: Luminescence assay results for cLuc gene with hUBC promoter</font></b>
 
<b><font size="+1">Figure 1: Luminescence assay results for cLuc gene with hUBC promoter</font></b>
  

Revision as of 00:49, 22 October 2021


hUBC-Kozak-cLuc

Cypridina luciferase is an enzyme that plays an important role in bioluminescence. In order to do this, it cleaves its substrate, vargulin, which produces a luminescent molecule. cLuc that is expressed in cells can be secreted into the culturing media, which allows it to be used as a dynamic reporter for the concentration of the compound of interest. This reporter can be used as a luminescence-based constitutive reporter to quantitatively and non-invasively assess cell behavior.

To test the gene expression of hUBC-cLuc, we transfected a plasmid containing this construct into HEK293T cells, and cell media samples were tested for luciferase data after 2 days using vargulin. Overall, the results demonstrate that the cLuc gene behaves as expected, as it induces luminescence activity when acting on its vargulin substrate.


800 px
Figure 1: Luminescence assay results for cLuc gene with hUBC promoter


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1018
    Illegal BglII site found at 1077
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]