Difference between revisions of "Part:BBa K4055533"

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===Experiment===
 
===Experiment===
[[File:T--Yucai_SZ--1.png|500px|thumb|center|Staining results of protein expressed by plasmid PT7-B0034-CsgA_linker_Mfp3spep, suggests that there's something sticking to the petri dish]]
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[[File:T--Yucai_SZ--rs.jpeg|600px|thumb|center|The staining results of a dish of CsgA-Mfp3S-pep bacteria with IPTG (Left) and a blank control dish of amilCP bacteria (Right)]]
[[File:T--Yucai_SZ--Juu10.png|500px|thumb|center|After 9 days of SBF culture, THE MFP-CSGA was scanned by electron microscopy (compared with the sample on day 0)]]
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Revision as of 00:49, 22 October 2021


CsgA-Mfp3S-pep

Biofilm structural proteins in E. coli fused with mussel foot protein(Mfp) analogs bestowed the engineered biofilms with HA mineralization-promoting and interfacial binding roles. When a blue light-induced strain was used to grow functional biofilms with controlled spatial and biomass density, living mineralized materials with patterning and gradient features could be generated following a benign biomimetic HA mineralization process.

an Mfp3S protein could initiate HA mineralization and promote interfacial adhesion.CsgA–Mfp fusion proteins comprising a CsgA domain (major protein component of the E. coli biofilm31) and a C-terminal Mfp serial fusion domain were constructed.

Moreover, examination of in vivo mineralization by TEM confirmed that CsgA–Mfp3S-pep-expressing biofilms triggered denser mineral formation with more apparent crystalline features after 5 d of min-eralization . These results thus highlight the roles of the Mfp3S-pep fusion protein in promoting HA mineral formation and crystallization.

Experiment

The staining results of a dish of CsgA-Mfp3S-pep bacteria with IPTG (Left) and a blank control dish of amilCP bacteria (Right)

After 9 days of SBF culture, THE MFP-CSGA was scanned by electron microscopy (compared with the sample on day 0).

SEM image showing the surface morphology of the biofilm (i.e., unmineralized). Scale bars:3μm
SEM image showing the surface morphology of the mineralized composite (mineralization 7d). Scale bars: 3μm

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]