Difference between revisions of "Part:BBa K3871004"

Latest revision as of 00:31, 22 October 2021

Description

mCherry is a red fluorescent protein used as a reporter derived from the fluorophore DsRed, which was originally isolated from Discosoma sea anemones. It is commonly used due to its colour and photostability compared to other monomeric fluorophores.

This part is a variant of mCherry, the sequence of which was modified to optimize its expression in Bacillus subtilis against E. coli using the Communique tool developed by the 2021 TAU Israel team. This specific variant was optimized based on typical decoding rate difference between B. subtilis and E. coli. You may read more about the optimization model here.

For more information on this part, please consult the 2021 TAU Israel wiki results page found here. Information about experimental procedures can be found here and here.

mCherry, TDR-D optimized for Bacillus subtilis

Assembly Compatibility:

10
INCOMPATIBLE WITH RFC[10]
Illegal EcoRI site found at 43
Illegal EcoRI site found at 91
Illegal EcoRI site found at 364
12
INCOMPATIBLE WITH RFC[12]
Illegal EcoRI site found at 43
Illegal EcoRI site found at 91
Illegal EcoRI site found at 364
21
INCOMPATIBLE WITH RFC[21]
Illegal EcoRI site found at 43
Illegal EcoRI site found at 91
Illegal EcoRI site found at 364
23
INCOMPATIBLE WITH RFC[23]
Illegal EcoRI site found at 43
Illegal EcoRI site found at 91
Illegal EcoRI site found at 364
25
INCOMPATIBLE WITH RFC[25]
Illegal EcoRI site found at 43
Illegal EcoRI site found at 91
Illegal EcoRI site found at 364
1000
COMPATIBLE WITH RFC[1000]

@@ Line 3: / Line 3: @@
    <p>
      mCherry is a red fluorescent protein used as a reporter derived from the
-     fluorophore DsRed, which wasthat originally isolated from
+     fluorophore DsRed, which was originally isolated from
      <i>Discosoma</i> sea anemones. It is commonly used due to its colour and
      photostability compared to other monomeric fluorophores.