Difference between revisions of "Part:BBa K3893000"
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<partinfo>BBa_K3893000 SequenceAndFeatures</partinfo>
 
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References
 
  
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[1] A. Yurovsky, M. R. Amin, J. Gardin, Y. Chen, S. Skiena, y B. Futcher, “Prokaryotic coding regions have little if any specific depletion of Shine-Dalgarno motifs”, PLOS ONE, vol. 13, núm. 8, p. e0202768, ago. 2018, doi: 10.1371/journal.pone.0202768.
 
  
 
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Revision as of 00:27, 22 October 2021


Shine Dalgarno for E coli

This sequence is part of the modular platform for producing dsRNAs in the proyect AgroBactory 593.

The Shine-Dalgarno (SD) is a sequence located 4 to 10 bases upstream of start codons and it is often 4 to 6 nucleotides long (most common: 5' AAGAG 3'). During translation, the SD sequence join to the 3' end of the 16S rRNA by base pairing with the anti-SD sequence (ASD); this mating helps the ribosomes to reach the right position of the start codon and therefore, translation can begin [1]. In synthetic biology, the SD sequence has been used to increase translation rate and as well as the yield of protein production [2]. On the other hand, for interference RNA (RNAi) assays, studies has shown that the SD sequence provides stability to the dsRNA molecules, preventing its degration [3]. For this reason, we decided to attach the SD sequence to our parts.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]