Difference between revisions of "Part:BBa K3893000"

Revision as of 00:22, 22 October 2021

Shine Dalgarno for E coli

This sequence is part of the modular platform for producing dsRNAs in the proyect AgroBactory 593.

The Shine-Dalgarno (SD) is a sequence located 4 to 10 bases upstream of start codons and it is often 4 to 6 nucleotides long (most common: 5' AAGAG 3'). During translation, the SD sequence join to the 3' end of the 16S rRNA by base pairing with the anti-SD sequence (ASD); this mating helps the ribosomes to reach the right position of the start codon and therefore, translation can begin [1]. In synthetic biology, the SD sequence has been used to increase translation rate and as well as the yield of protein production [2]. On the other hand, for interference RNA (RNAi) assays, studies has shown that the SD sequence provides stability to the dsRNA molecules, preventing its degration [3]. For this reason, we decided to attach the SD sequence to our parts.

References

[1] A. Yurovsky, M. R. Amin, J. Gardin, Y. Chen, S. Skiena, y B. Futcher, “Prokaryotic coding regions have little if any specific depletion of Shine-Dalgarno motifs”, PLOS ONE, vol. 13, núm. 8, p. e0202768, ago. 2018, doi: 10.1371/journal.pone.0202768.

Sequence and Features