Difference between revisions of "Part:BBa K3805329"
 
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We combined three kinds of Human insulin proteinogen(https://parts.igem.org/Part:BBa_K3805429, https://parts.igem.org/Part:BBa_K3805001, https://parts.igem.org/Part:BBa_K3805001) with AraC(https://parts.igem.org/Part:BBa_I0500), let Human insulin proteinogen sequence affect the expression of AraC. Here we take human insulin proteinogen(Normal) as an example.
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We combined three kinds of proinsulin Normal<a href = "https://parts.igem.org/Part:BBa_K3805429"> (BBa_K3805429)</a>, Mutation Ⅰ<a href = "https://parts.igem.org/Part:BBa_K3805666"> (BBa_K3805666)</a>, Mutation Ⅱ<a href = "https://parts.igem.org/Part:BBa_K3805001"> (BBa_K3805001)</a> with araC <a href = "https://parts.igem.org/Part:BBa_I0500">(BBa_I0500)</a>, letting proinsulin sequence affect the expression of araC. Here we take proinsulin(Normal) as an example.
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Latest revision as of 00:16, 22 October 2021


Fusion protein with proinsulin and araC

We combined three kinds of proinsulin Normal (BBa_K3805429), Mutation Ⅰ (BBa_K3805666), Mutation Ⅱ (BBa_K3805001) with araC (BBa_I0500), letting proinsulin sequence affect the expression of araC. Here we take proinsulin(Normal) as an example.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 1487
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 1426
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 1261
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI site found at 1243