Difference between revisions of "Part:BBa K4088898"

 
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<partinfo>BBa_K4088898 short</partinfo>
 
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Cas13a is a classic RNA-targeting nuclease that is widely used in a variety of diagnostics methods. It is a very specific variant of RNA detection, it can be customized to recognize single nucleotides on the target RNA. dCas13a is a catalytically dead LwaCas13a enzyme.
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Cas13a is a classic RNA-targeting nuclease that is widely used in a variety of diagnostics methods. It is a very specific variant of RNA detection, it can be customized to recognize single nucleotides on the target RNA. dCas13a is a catalytically dead LwaCas13a enzyme.<br/>
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For more information, see <partinfo>BBa_K4088891</partinfo>.
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Revision as of 00:08, 22 October 2021


dCas13a

Cas13a is a classic RNA-targeting nuclease that is widely used in a variety of diagnostics methods. It is a very specific variant of RNA detection, it can be customized to recognize single nucleotides on the target RNA. dCas13a is a catalytically dead LwaCas13a enzyme.
For more information, see BBa_K4088891.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 622
    Illegal PstI site found at 601
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 622
    Illegal PstI site found at 601
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 622
    Illegal BglII site found at 924
    Illegal BglII site found at 1689
    Illegal BglII site found at 1956
    Illegal BglII site found at 2166
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 622
    Illegal PstI site found at 601
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 622
    Illegal PstI site found at 601
  • 1000
    COMPATIBLE WITH RFC[1000]