Difference between revisions of "Part:BBa K3788019:Design"

 
 
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===Design Notes===
 
===Design Notes===
−
XbaI restriction site was delated
 
  
  
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===Source===
 
===Source===
  
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colA sequence was given by LISM laboratory from CNRS. RFP coding sequence was taken corresponding to Part:BBa_E1010
 
  
  
  
 
===References===
 
===References===
 +
<p> - Cascales, E. et al. Colicin biology. <i>Microbiol. Mol. Biol. Rev. MMBR</i><b>71</b>, 158–229 (2007). </p>
 +
<p> - Cavard, D. Role of Cal, the colicin A lysis protein, in two steps of colicin A release and in the interaction with colicin A-porin complexes. <i>Microbiol. Read. Engl</i><b>. 150</b>, 3867–3875 (2004).</p>
 +
<p> - Lloubes, R., Baly, D. & Lazdunski, C. The promoters of the genes for colicin production, release and immunity in the ColA plasmid: effects of convergent transcription and Lex A protein. <i>Nucleic Acids Res </i>. <b>14</b>, 2621–2636 (1986). </p>
 +
<a class=”bg-transparent” href=”https://www.prodoric.de/vfp.”>VirtualFootprint - PRODORIC</a>

Latest revision as of 00:06, 22 October 2021


Expression of GFP and of the timer device under the control of the LexA-controlled promoter


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 1528
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1848
    Illegal BamHI site found at 1521
    Illegal XhoI site found at 1397
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 1597
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 867
    Illegal SapI.rc site found at 1351


Design Notes

Source

References

- Cascales, E. et al. Colicin biology. Microbiol. Mol. Biol. Rev. MMBR71, 158–229 (2007).

- Cavard, D. Role of Cal, the colicin A lysis protein, in two steps of colicin A release and in the interaction with colicin A-porin complexes. Microbiol. Read. Engl. 150, 3867–3875 (2004).

- Lloubes, R., Baly, D. & Lazdunski, C. The promoters of the genes for colicin production, release and immunity in the ColA plasmid: effects of convergent transcription and Lex A protein. Nucleic Acids Res . 14, 2621–2636 (1986).

<a class=”bg-transparent” href=”https://www.prodoric.de/vfp.”>VirtualFootprint - PRODORIC</a>