Difference between revisions of "Part:BBa K3792003"
| Line 1: | Line 1: | ||
{{FSU/ChartsCSS}} | {{FSU/ChartsCSS}} | ||
| + | <html> | ||
<style> | <style> | ||
#modular-promoter-library-chart.column { | #modular-promoter-library-chart.column { | ||
| Line 20: | Line 21: | ||
} | } | ||
</style> | </style> | ||
| + | </html> | ||
__NOTOC__ | __NOTOC__ | ||
Revision as of 23:40, 21 October 2021
BIOFAB apFAB40 Measurement Device
apFAB40 is a strong promoter taken from the BIOFAB collection. This device uses apFAB40 to express mRFP1, a red fluorescent protein. The apFAB40 test device consists of apFAB40, an RBS, the coding sequence for mRFP1, and a terminator.
This test device and two others with BIOFAB promoters of different strengths were each assembled into the pSB1K3 plasmid and then transformed into E. coli NEB 5 alpha.
The fluorescence of the cells was measured using flow cytometry. By comparing the relative fluorescence, we can determine which promoters caused higher levels of expression of mRFP1.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 614
Illegal AgeI site found at 726 - 1000COMPATIBLE WITH RFC[1000]