Difference between revisions of "Part:BBa K3792003"
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{{FSU/ChartsCSS}}
 
{{FSU/ChartsCSS}}
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<style>
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#modular-promoter-library-chart.column {
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  height: 400px;
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  max-width: 100% !important;
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  margin: 0 auto;
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  --labels-size: 0.5rem;
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}
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#modular-promoter-library-chart tr {
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  transition-duration: 0.3s;
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}
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#modular-promoter-library-chart tr:hover {
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  background-color: rgba(0, 0, 0, 0.2);
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}
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#modular-promoter-library-chart tr:hover th {
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  background-color: rgba(0, 0, 0, 0.4);
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  color: #fff;
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}
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</style>
  
 
__NOTOC__
 
__NOTOC__

Revision as of 23:39, 21 October 2021

<style>

  1. modular-promoter-library-chart.column {
 height: 400px;
 max-width: 100% !important;
 margin: 0 auto;
 --labels-size: 0.5rem;

}

  1. modular-promoter-library-chart tr {
 transition-duration: 0.3s;

}

  1. modular-promoter-library-chart tr:hover {
 background-color: rgba(0, 0, 0, 0.2);

}

  1. modular-promoter-library-chart tr:hover th {
 background-color: rgba(0, 0, 0, 0.4);
 color: #fff;

} </style>


BIOFAB apFAB40 Measurement Device

apFAB40 is a strong promoter taken from the BIOFAB collection. This device uses apFAB40 to express mRFP1, a red fluorescent protein. The apFAB40 test device consists of apFAB40, an RBS, the coding sequence for mRFP1, and a terminator.

This test device and two others with BIOFAB promoters of different strengths were each assembled into the pSB1K3 plasmid and then transformed into E. coli NEB 5 alpha.

The fluorescence of the cells was measured using flow cytometry. By comparing the relative fluorescence, we can determine which promoters caused higher levels of expression of mRFP1.

iGEM: BBa_M36303
BIOFAB: apFAB46
Strength: 897897
iGEM: BBa_K2832101
BIOFAB: apFAB70
Strength: 866.7866.7
iGEM: BBa_K2832102
BIOFAB: apFAB71
Strength: 866866
iGEM: BBa_K2832103
BIOFAB: apFAB61
Strength: 857857


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 614
    Illegal AgeI site found at 726
  • 1000
    COMPATIBLE WITH RFC[1000]