Difference between revisions of "Part:BBa K3792003"
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The fluorescence of the cells was measured using flow cytometry. By comparing the relative fluorescence, we can determine which promoters caused higher levels of expression of mRFP1.
 
The fluorescence of the cells was measured using flow cytometry. By comparing the relative fluorescence, we can determine which promoters caused higher levels of expression of mRFP1.
  
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<table id="modular-promoter-library-chart" class="charts-css column hide-data show-primary-axis show-10-secondary-axes">
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      <tr>
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        <td style="--size: calc(897/900)"><span class="tooltip">iGEM: [https://parts.igem.org/Part:BBa_M36303 BBa_M36303]<br>BIOFAB: apFAB46<br>Strength: 897</span><span class="data">897</span></td>
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      </tr>
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      <tr>
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      <td style="--size: calc(866.7/900)"><span class="tooltip">iGEM: [https://parts.igem.org/Part:BBa_K2832101 BBa_K2832101]<br>BIOFAB: apFAB70<br>Strength: 866.7</span><span class="data">866.7</span></td>
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      </tr>
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      <tr>
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        <td style="--size: calc(866/900)"><span class="tooltip">iGEM: [https://parts.igem.org/Part:BBa_K2832102 BBa_K2832102]<br>BIOFAB: apFAB71<br>Strength: 866</span><span class="data">866</span></td>
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      </tr>
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      <tr>     
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        <td style="--size: calc(857/900)"><span class="tooltip">iGEM: [https://parts.igem.org/Part:BBa_K2832103 BBa_K2832103]<br>BIOFAB: apFAB61<br>Strength: 857</span><span class="data">857</span></td>
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    </tr>
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  </table>
  
  

Revision as of 23:37, 21 October 2021


BIOFAB apFAB40 Measurement Device

apFAB40 is a strong promoter taken from the BIOFAB collection. This device uses apFAB40 to express mRFP1, a red fluorescent protein. The apFAB40 test device consists of apFAB40, an RBS, the coding sequence for mRFP1, and a terminator.

This test device and two others with BIOFAB promoters of different strengths were each assembled into the pSB1K3 plasmid and then transformed into E. coli NEB 5 alpha.

The fluorescence of the cells was measured using flow cytometry. By comparing the relative fluorescence, we can determine which promoters caused higher levels of expression of mRFP1.

iGEM: BBa_M36303
BIOFAB: apFAB46
Strength: 897897
iGEM: BBa_K2832101
BIOFAB: apFAB70
Strength: 866.7866.7
iGEM: BBa_K2832102
BIOFAB: apFAB71
Strength: 866866
iGEM: BBa_K2832103
BIOFAB: apFAB61
Strength: 857857


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 614
    Illegal AgeI site found at 726
  • 1000
    COMPATIBLE WITH RFC[1000]