Difference between revisions of "Part:BBa K3722002"

 
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Monooxygenase hydroxylates the C3 position of the indole ring and forms 6-Br-indoxyl.
 
Monooxygenase hydroxylates the C3 position of the indole ring and forms 6-Br-indoxyl.
  
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===Usage and Biology===
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===Biological function===
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Monooxygenase from Methylophaga aminisulfidivorans (MaFMO). And flavin is used as a cofactor when MaFMO hydroxylates the C3 position of the indole ring and forms indigo. Indigo has a maximum absorption peak at 606nm.
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===Instruction===
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The coding sequence of target gene was inserted into an expression vectors with BBa_K3722000 to obtain BBa_K3722011. We transformed the constructed plasmid into E. coli BL21 (DE3) to verify its successful heterologous expression.
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Revision as of 23:22, 21 October 2021


MaFMO

Monooxygenase hydroxylates the C3 position of the indole ring and forms 6-Br-indoxyl.


Biological function

Monooxygenase from Methylophaga aminisulfidivorans (MaFMO). And flavin is used as a cofactor when MaFMO hydroxylates the C3 position of the indole ring and forms indigo. Indigo has a maximum absorption peak at 606nm.


Instruction

The coding sequence of target gene was inserted into an expression vectors with BBa_K3722000 to obtain BBa_K3722011. We transformed the constructed plasmid into E. coli BL21 (DE3) to verify its successful heterologous expression.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]