Difference between revisions of "Part:BBa K4015017"
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<partinfo>BBa_K4015017 short</partinfo> | <partinfo>BBa_K4015017 short</partinfo> | ||
− | CotA-NusA- | + | CotA-NusA-Lcp1VH2-HlyA is composed of four basic parts, CotA, NusA, Lcp1VH2, and HlyA. The composition enables the secretion of Lcp with enhanced solubility and expression rate, with clear indication. |
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Latest revision as of 23:10, 21 October 2021
CotA-NusA-Lcp1VH2-HlyA
CotA-NusA-Lcp1VH2-HlyA is composed of four basic parts, CotA, NusA, Lcp1VH2, and HlyA. The composition enables the secretion of Lcp with enhanced solubility and expression rate, with clear indication.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 6
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 4440
Illegal XhoI site found at 5070
Illegal XhoI site found at 6595
Illegal XhoI site found at 6749 - 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 4689
Illegal NgoMIV site found at 5047
Illegal NgoMIV site found at 6241
Illegal NgoMIV site found at 6563
Illegal AgeI site found at 2713 - 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI site found at 4674
Illegal BsaI.rc site found at 186
Illegal BsaI.rc site found at 1631
Illegal BsaI.rc site found at 1849
Illegal BsaI.rc site found at 6144
Illegal SapI.rc site found at 4662