Difference between revisions of "Part:BBa K3736001"
| Line 5: | Line 5: | ||
constitutive promoter+ FourU + luxR + Terminator*2 | constitutive promoter+ FourU + luxR + Terminator*2 | ||
| + | [[File:T--NCTU_Formosa--stath seqA.png|500px|thumb|center|'''Figure 1. Quorum sensing sequence | ||
| + | ]] | ||
= Gene Construct of DenTeeth = | = Gene Construct of DenTeeth = | ||
| Line 10: | Line 12: | ||
  We incorporate the whole part into <i>E. coli</i> BL21(DE3). We did colony PCR and digest to check its genotype. |   We incorporate the whole part into <i>E. coli</i> BL21(DE3). We did colony PCR and digest to check its genotype. | ||
| − | [[File:T--NCTU_Formosa--stath seq.png|300px|thumb|center|'''Figure | + | [[File:T--NCTU_Formosa--stath seq.png|300px|thumb|center|'''Figure 2. Quorum sensing sequence + Restoration sequence + Sterilization sequence. M :1kb DNA ladder, 1 : Constitutive promoter + RBS + LuxR + Term. +Term. + Ptet + RBS + BMP2 + RBS + STATH + RBS + GFP + Term. + Term. + Plux + RBS + LL-37 + RBS + mRFP + RBS + tetR + Term. + Term. (4685 b.p.)</i> |
]] | ]] | ||
Revision as of 22:36, 21 October 2021
constitutive promoter+ FourU + luxR + Terminator*2
constitutive promoter+ FourU + luxR + Terminator*2
Gene Construct of DenTeeth
We incorporate the whole part into E. coli BL21(DE3). We did colony PCR and digest to check its genotype.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 7
Illegal NheI site found at 30 - 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 890
Illegal NgoMIV site found at 1011 - 1000COMPATIBLE WITH RFC[1000]