Difference between revisions of "Part:BBa K3725010:Experience"
 
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<center>[[File:T--Lambert GA--PhytoIPTGFluoresenceData.jpeg|500px]]</center>
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<center>[[File:T--Lambert GA--PhytoIPTGFluorescenceDataGraph.jpeg|500px]]</center>
 
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<center><I>Figure 2: This graph compares the mean fluorescence per optical density values of the P. cryptogea toehold switch, positive control (pUC19), and the dual plasmid. The toehold switch and positive control have very low fluorescence per optical density values, whereas the dual plasmid has a higher fluorescence per optical density value, that is significantly different from the other two groups..</I></center>
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<center><I>Figure 2. Mean fluorescence/OD of IPTG-induced Phytophthora dual plasmid transformation compared to toehold and pUC19 with SEM error bars. DP stands for dual plasmid, TH stands for toehold only. Ran at gain of 60.</I></center>
 
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Latest revision as of 22:35, 21 October 2021


Experience
The T7 Phytophthora Trigger (BBa_K3725040) and the Phytophthora Toehold w/ GFP Reporter are intended to be compatible with each other and used in conjunction. To test the compatibility of the trigger sequence with the toehold sequence, a dual-plasmid transformation was performed, and fluorescence was measured in comparison with cells transformed with only the toehold part and pUC19 (positive control). All samples were divided by optical density to obtain a standardized unit. The fluorescence per optical density unit measurement of cells transformed with both the toehold and trigger sequences was greater and had a statistically significant difference in comparison to cells transformed with only the toehold part and pUC19 plasmid.

T--Lambert GA--PhytoIPTGFluorescenceDataGraph.jpeg


Figure 2. Mean fluorescence/OD of IPTG-induced Phytophthora dual plasmid transformation compared to toehold and pUC19 with SEM error bars. DP stands for dual plasmid, TH stands for toehold only. Ran at gain of 60.



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