Difference between revisions of "Part:BBa K3736001"
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constitutive promoter+ FourU + luxR + Terminator*2  
 
constitutive promoter+ FourU + luxR + Terminator*2  
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= Gene Construct of DenTeeth =
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&#8195;&#8195;We incorporate the whole part into <i>E. coli</i> BL21(DE3). We did colony PCR and digest to check its genotype.
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[[File:T--NCTU_Formosa--stath seq.png|300px|thumb|center|'''Figure 1.  Quorum sensing sequence + Restoration sequence + Sterilization sequence. M :1kb DNA ladder, 1 : Constitutive promoter + RBS + LuxR + Term. +Term. + Ptet + RBS + BMP2 + RBS + STATH + RBS + GFP + Term. + Term.  + Plux + RBS + LL-37 + RBS + mRFP + RBS + tetR + Term. + Term. (4685 b.p.)</i>
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<!-- Add more about the biology of this part here
 
<!-- Add more about the biology of this part here

Revision as of 22:32, 21 October 2021


constitutive promoter+ FourU + luxR + Terminator*2

constitutive promoter+ FourU + luxR + Terminator*2


Gene Construct of DenTeeth

  We incorporate the whole part into E. coli BL21(DE3). We did colony PCR and digest to check its genotype.

Figure 1. Quorum sensing sequence + Restoration sequence + Sterilization sequence. M :1kb DNA ladder, 1 : Constitutive promoter + RBS + LuxR + Term. +Term. + Ptet + RBS + BMP2 + RBS + STATH + RBS + GFP + Term. + Term. + Plux + RBS + LL-37 + RBS + mRFP + RBS + tetR + Term. + Term. (4685 b.p.)</i>


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 890
    Illegal NgoMIV site found at 1011
  • 1000
    COMPATIBLE WITH RFC[1000]