Difference between revisions of "Part:BBa K3791004"

 
 
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<partinfo>BBa_K3791004 short</partinfo>
 
<partinfo>BBa_K3791004 short</partinfo>
  
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This part contains the spacer sequence used to target a specific DNA fragment of a certain spectinomycin-resistant gene. It must be used in combination with the proper repeat in order to conform the whole gRNA functionality. More specifically, we placed it in a plasmid after the repeat sequence, already cataloged in the Parts registry. In our case, we used it for activating LbCas12a protein, so the repeat sequence appropriate for our design was [https://parts.igem.org/wiki/index.php?title=Part:BBa_K2927006 BBa_K2927006]. The whole gRNA construct containing both spacer and repeat is available in [link (2)] and the same one but with the required promoter and terminator is available in [link (3)].
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This part contains the spacer sequence used to target a specific DNA fragment of a certain spectinomycin-resistant gene. It must be used in combination with the proper repeat in order to conform the whole gRNA functionality [1]. More specifically, we placed it in a plasmid after the repeat sequence, already cataloged in the Parts registry. In our case, we used it for activating LbCas12a protein, so the repeat sequence appropriate for our design was <html><a href="https://parts.igem.org/Part:BBa_K2927006" target="_blank" onmouseover="this.style.color='#ff724a';" onmouseout="this.style.color='#ff9515';" style="color: #ff9515; text-decoration: none;">BBa_K2927006</a></html>. The whole gRNA construct containing both spacer and repeat is available in <html><a href="https://parts.igem.org/Part:BBa_K3791009" target="_blank" onmouseover="this.style.color='#ff724a';" onmouseout="this.style.color='#ff9515';" style="color: #ff9515; text-decoration: none;">BBa_K3791009</a></html> and the same one but with the required promoter and terminator is available in <html><a href="https://parts.igem.org/Part:BBa_K3791014" target="_blank" onmouseover="this.style.color='#ff724a';" onmouseout="this.style.color='#ff9515';" style="color: #ff9515; text-decoration: none;">BBa_K3791014</a></html>.
  
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===Usage and Biology===
 
  
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===Usage, Biology and Characterization===
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<span class='h3bb'>Sequence and Features</span>
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As this part is a simplification of our final construct, usage, biology and characterization is explained on both the preliminary gRNA construct part <html><a href="https://parts.igem.org/Part:BBa_K3791014" target="_blank" onmouseover="this.style.color='#ff724a';" onmouseout="this.style.color='#ff9515';" style="color: #ff9515; text-decoration: none;">BBa_K3791014</a></html> and the Efficient gRNA construct part <html><a href="https://parts.igem.org/Part:BBa_K3791024" target="_blank" onmouseover="this.style.color='#ff724a';" onmouseout="this.style.color='#ff9515';" style="color: #ff9515; text-decoration: none;">BBa_K3791024</a></html>
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<span class='h3bb'><b>Sequence and Features</b></span>
 
<partinfo>BBa_K3791004 SequenceAndFeatures</partinfo>
 
<partinfo>BBa_K3791004 SequenceAndFeatures</partinfo>
  

Latest revision as of 22:20, 21 October 2021


Spacer gRNA Spectinomycin

This part contains the spacer sequence used to target a specific DNA fragment of a certain spectinomycin-resistant gene. It must be used in combination with the proper repeat in order to conform the whole gRNA functionality [1]. More specifically, we placed it in a plasmid after the repeat sequence, already cataloged in the Parts registry. In our case, we used it for activating LbCas12a protein, so the repeat sequence appropriate for our design was BBa_K2927006. The whole gRNA construct containing both spacer and repeat is available in BBa_K3791009 and the same one but with the required promoter and terminator is available in BBa_K3791014.


Usage, Biology and Characterization

As this part is a simplification of our final construct, usage, biology and characterization is explained on both the preliminary gRNA construct part BBa_K3791014 and the Efficient gRNA construct part BBa_K3791024


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]