Difference between revisions of "Part:BBa K4044003:Design"
 
Line 7: Line 7:
  
 
===Design Notes===
 
===Design Notes===
−
Codon optimization of the nucleotide sequence for efficient gene expression in E.coli was performed using GENEWIZ codon optimization tool.
+
Codon optimization of the nucleotide sequence for efficient gene expression in ''E. coli'' was performed using GENEWIZ codon optimization tool.
  
  
  
 
===Source===
 
===Source===
−
BcLOV4 protein sequence was taken from https://www.uniprot.org/uniprot/A0A384J9J8 and codon optimized for E. coli.
+
BcLOV4 protein sequence was taken from https://www.uniprot.org/uniprot/A0A384J9J8 and codon optimized for ''E. coli''.
  
−
Source organism: Botrytis cinerea
+
Source organism: ''Botrytis cinerea''
  
 
===References===
 
===References===

Latest revision as of 22:18, 21 October 2021


BcLOV4


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 354
    Illegal PstI site found at 598
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 354
    Illegal PstI site found at 598
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 354
    Illegal PstI site found at 598
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 354
    Illegal PstI site found at 598
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Codon optimization of the nucleotide sequence for efficient gene expression in E. coli was performed using GENEWIZ codon optimization tool.


Source

BcLOV4 protein sequence was taken from https://www.uniprot.org/uniprot/A0A384J9J8 and codon optimized for E. coli.

Source organism: Botrytis cinerea

References