Difference between revisions of "Part:BBa K3725150"

 
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====DESIGN====
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In an attempt to increase the sensitivity of BBa_3725130 to extracellular phosphate, we designed part BBa_K3725150, a phosphate-sensitive biosensor with modified promoter BBa_K3725140 and GFP reporter. The new promoter utilizes a potentially more efficient consensus sequence by directly mutating the RNA polymerase binding site. The mutation affects the construct’s ability to transcribe downstream GFP, thus increasing the sensitivity of the construct and potentially creating an enhanced characterization curve.
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<span class='h3bb'>Sequence and Features</span>
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Revision as of 22:03, 21 October 2021


PhoB Redesigned Mutated Consensus Promoter w/ GFP Reporter

DESIGN

In an attempt to increase the sensitivity of BBa_3725130 to extracellular phosphate, we designed part BBa_K3725150, a phosphate-sensitive biosensor with modified promoter BBa_K3725140 and GFP reporter. The new promoter utilizes a potentially more efficient consensus sequence by directly mutating the RNA polymerase binding site. The mutation affects the construct’s ability to transcribe downstream GFP, thus increasing the sensitivity of the construct and potentially creating an enhanced characterization curve.



Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 1161