Difference between revisions of "Part:BBa K3799010"

Latest revision as of 21:59, 21 October 2021

AIP-1 sensor of S.aureus under pBAD promoter

This part consist of the AIP-1 sensing and signal transduction system of S aureus(BBa_I746001) under pveg,constitutive promoter, of B.subtilis(BBa_K143012).

The AgrC-AgrA is a two component signal transduction system which senses the AIP-1 molecule secreted by S.aureus.AgrC encodes a transmembrane histidine kinase receptor which binds to exogenous AIP-1 secreted by other S.aureus which inturn phosphorylates intracellular AgrA which acts as a signalling molecule which inturn activates the P2 and P3 promoter involved in quorum sensing,

This part can be used to replicate the two component signal transduction system from S.aureus into other host such as B.subtilis and Ecoli

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
INCOMPATIBLE WITH RFC[12]
Illegal NheI site found at 125
21
INCOMPATIBLE WITH RFC[21]
Illegal BglII site found at 513
Illegal BamHI site found at 65
Illegal BamHI site found at 1397
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
COMPATIBLE WITH RFC[1000]

@@ Line 3: / Line 3: @@
 <partinfo>BBa_K3799010 short</partinfo>
-This part consist of the AIP-1 sensing and signal transduction system of S aureus(BBa_I746101) under pveg,constitutive promoter, of B.subtilis(BBa_k143012).
+This part consist of the AIP-1 sensing and signal transduction system of S aureus([https://parts.igem.org/Part:BBa_I746001 BBa_I746001]) under pveg,constitutive promoter, of B.subtilis([https://parts.igem.org/Part:BBa_K143012 BBa_K143012]).
 The AgrC-AgrA is a two component signal transduction system which senses the AIP-1 molecule secreted by S.aureus.AgrC encodes a transmembrane histidine kinase receptor which binds to exogenous AIP-1 secreted by other S.aureus which inturn phosphorylates intracellular AgrA which acts as a signalling molecule which inturn activates the P2 and P3 promoter involved in quorum sensing,