Difference between revisions of "Part:BBa K3783003:Experience"
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how you used this part and how it worked out. | how you used this part and how it worked out. | ||
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| + | <img src="https://2021.igem.org/wiki/images/b/b0/T--OhioState--LALAssayGraph.png" class="generalimage" alt="LAL Assay Graph"> | ||
| + | <figcaption style="text-align:center">Figure 2. Endotoxin Units of Construct Lysates via LAL Assay</figcaption> | ||
| + | </figure> | ||
| + | <p>The 2021 OhioState team characterized this biobrick using a Pierce Chromogenic Endotoxin Quantification Kit from ThermoFisher. The results were treated with Limulus Amebocyte Lysate which binds endotoxin and then attached to a chromogenic substrate which allowed us to quantify our results with provided standards. Cultures diluted to the -5 were used to quantify endotoxin concentrations. A control without any anti-lipid A proteins was also used as a comparison. There was a noticeable decrease in bound endotoxin. | ||
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===User Reviews=== | ===User Reviews=== | ||
Latest revision as of 21:54, 21 October 2021
This experience page is provided so that any user may enter their experience using this part.
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how you used this part and how it worked out.
OhioState Application
The 2021 OhioState team characterized this biobrick using a Pierce Chromogenic Endotoxin Quantification Kit from ThermoFisher. The results were treated with Limulus Amebocyte Lysate which binds endotoxin and then attached to a chromogenic substrate which allowed us to quantify our results with provided standards. Cultures diluted to the -5 were used to quantify endotoxin concentrations. A control without any anti-lipid A proteins was also used as a comparison. There was a noticeable decrease in bound endotoxin.
User Reviews
UNIQ6f03e090b29118db-partinfo-00000001-QINU UNIQ6f03e090b29118db-partinfo-00000002-QINU