Difference between revisions of "Part:BBa K3725110"
Line 4: Line 4:
  
 
====DESIGN====
 
====DESIGN====
−
The inducible promoter BBa_K1682012, created by 2015 HKUST-Rice iGEM, was originally made to control the expression of the PhoA alkaline phosphatase gene following activation by the phosphorylated PhoB transcription factor. By replacing the downstream PhoA gene with GFP,  the part expresses fluorescence in the presence of low levels of extracellular phosphate.  
+
The inducible promoter BBa_K1682012, created by 2015 HKUST-Rice iGEM, was originally made to control the expression of the PhoA alkaline phosphatase gene following activation by the phosphorylated PhoB transcription factor.  
  
 
====EXPERIENCE====
 
====EXPERIENCE====
 
Utilizing the modified phosphate range and characterization protocol (See: Modified Protocol under Pho), we characterized the BBa_K3725110 phosphate sensor. However, the characterization data (Figure 1) yielded insignificant levels of fluorescence, thus leading us to conclude that the promoter is not sensitive enough to extracellular phosphate levels.  
 
Utilizing the modified phosphate range and characterization protocol (See: Modified Protocol under Pho), we characterized the BBa_K3725110 phosphate sensor. However, the characterization data (Figure 1) yielded insignificant levels of fluorescence, thus leading us to conclude that the promoter is not sensitive enough to extracellular phosphate levels.  
−
 
−
 
  
 
[[File:Fjasksdanfnkcnfkncjak.png|thumb|center|800px|Figure 5: Characterization curve for BBa_K3725110 for phosphate concentrations between 0μM and 100μM. ]]
 
[[File:Fjasksdanfnkcnfkncjak.png|thumb|center|800px|Figure 5: Characterization curve for BBa_K3725110 for phosphate concentrations between 0μM and 100μM. ]]

Revision as of 21:53, 21 October 2021


PhoA Promoter with GFP Reporter

DESIGN

The inducible promoter BBa_K1682012, created by 2015 HKUST-Rice iGEM, was originally made to control the expression of the PhoA alkaline phosphatase gene following activation by the phosphorylated PhoB transcription factor.

EXPERIENCE

Utilizing the modified phosphate range and characterization protocol (See: Modified Protocol under Pho), we characterized the BBa_K3725110 phosphate sensor. However, the characterization data (Figure 1) yielded insignificant levels of fluorescence, thus leading us to conclude that the promoter is not sensitive enough to extracellular phosphate levels.

Figure 5: Characterization curve for BBa_K3725110 for phosphate concentrations between 0μM and 100μM.


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 741