Difference between revisions of "Part:BBa K3739111"
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===Usage=== | ===Usage=== | ||
By codon optimization, the sequence suitable for expression in Vibrio Natriegens was constructed. Adding T7-RBS and terminator,construct new composite part BBa_K3739111.We transformed the constructed plasmid into Vibrio Natriegens to verify its successful heterologous expression. | By codon optimization, the sequence suitable for expression in Vibrio Natriegens was constructed. Adding T7-RBS and terminator,construct new composite part BBa_K3739111.We transformed the constructed plasmid into Vibrio Natriegens to verify its successful heterologous expression. | ||
| + | [[File:T--XMU-China--K3739111.png]] | ||
===Reference=== | ===Reference=== | ||
Revision as of 21:52, 21 October 2021
K525998(T7-RBS)-Aly01-PPO-his-B0010
Aly01 is a signal peptide from the alginate lyase of V.natriegens and PPO, a tyrosinase able to oxidize polyphenol, is to weaken the mfp glue.his-tag is used to be detected by anti-his antibody and purify the his-linking fusion
Biology
Aly01 is a signal peptide from the alginate lyase of V.natriegens. PPO, a tyrosinase able to oxidize polyphenol, is to weaken the mussel foot protein glue. his-tag is used to be detected by anti-his antibody and purify the his-linking fusion.
Usage
By codon optimization, the sequence suitable for expression in Vibrio Natriegens was constructed. Adding T7-RBS and terminator,construct new composite part BBa_K3739111.We transformed the constructed plasmid into Vibrio Natriegens to verify its successful heterologous expression.
Reference
1. Holten-Andersen, N.; Waite, J. H., Mussel-designed protective coatings for compliant substrates. J Dent Res 2008, 87 (8), 701-9.
2. Kim, E.; Dai, B.; Qiao, J. B.; Li, W.; Fortner, J. D.; Zhang, F., Microbially Synthesized Repeats of Mussel Foot Protein Display Enhanced Underwater Adhesion. ACS Appl Mater Interfaces 2018, 10 (49), 43003-43012.
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]