Difference between revisions of "Part:BBa K3722001"
 
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===Biology===
 
===Biology===
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Flavin reductase catalyzes the reduction of free flavin using NADH to produce free reduced flavin. The reduction of riboflavin by NADPH catalyzed by flavin reductase has been studied by static and rapid kinetics absorption spectroscopies. Static absorption spectroscopy experiments revealed that, in the presence of riboflavin and reduced pyridine nucleotide, flavin reductase stabilizes, although to a small extent, a charge-transfer complex of NADP+ and reduced riboflavin[1].
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Flavin reductase catalyzes the reduction of free flavin using NADH to produce free reduced flavin. The reduction of riboflavin by NADPH catalyzed by flavin reductase has been studied by static and rapid kinetics absorption spectroscopies. Static absorption spectroscopy experiments revealed that, in the presence of riboflavin and reduced pyridine nucleotide, flavin reductase stabilizes, although to a small extent, a charge-transfer complex of NADP+ and reduced riboflavin<ref>Nivière V, Vanoni MA, Zanetti G, Fontecave M. Reaction of the NAD(P)H:flavin oxidoreductase from Escherichia coli with NADPH and riboflavin: identification of intermediates. Biochemistry. 1998 Aug 25;37(34):11879-87. doi: 10.1021/bi980396f. PMID: 9718311.</ref>.
  
  
 
===Usage===
 
===Usage===
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Here, we used BBa_K3722004 and to construct the expression system and obtained the part BBa_K3722006, which may achieve surface display of SttH on our engineered bacteria. Due to the limited time, we did not characterize this part. As a result, there is a lack of data about this part.
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Here, we used <partinfo>BBa_K3722004</partinfo> and to construct the expression system and obtained the part <partinfo>BBa_K3722006</partinfo>, it can increase the solubility of SttH on our engineered bacteria. Due to the limited time, we only constructed part <partinfo>BBa_K3722009</partinfo> and <partinfo>BBa_K3722010</partinfo>, verified the protein expression by SDS PAGE analysis. As a result, there is a lack of quantized data about this part.
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    <figure>
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        <img src="https://2021.igem.org/wiki/images/2/2b/T--NWU-CHINA-A--part01.png" width="100%" style="float:center">
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        <figcaption>
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        <p style="font-size:1rem">
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        </p>
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        </figcaption>
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    </figure>
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</html>
  
  
 
===References===
 
===References===
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[1] Nivière V, Vanoni MA, Zanetti G, Fontecave M. Reaction of the NAD(P)H:flavin oxidoreductase from Escherichia coli with NADPH and riboflavin: identification of intermediates. Biochemistry. 1998 Aug 25;37(34):11879-87. doi: 10.1021/bi980396f. PMID: 9718311.
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<references/>
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Latest revision as of 21:28, 21 October 2021


Fre(flavin reductase)

Flavin reductase (Fre) regenerates FAD to FADH2 using NAD(P)H.


Biology

Flavin reductase catalyzes the reduction of free flavin using NADH to produce free reduced flavin. The reduction of riboflavin by NADPH catalyzed by flavin reductase has been studied by static and rapid kinetics absorption spectroscopies. Static absorption spectroscopy experiments revealed that, in the presence of riboflavin and reduced pyridine nucleotide, flavin reductase stabilizes, although to a small extent, a charge-transfer complex of NADP+ and reduced riboflavin[1].


Usage

Here, we used BBa_K3722004 and to construct the expression system and obtained the part BBa_K3722006, it can increase the solubility of SttH on our engineered bacteria. Due to the limited time, we only constructed part BBa_K3722009 and BBa_K3722010, verified the protein expression by SDS PAGE analysis. As a result, there is a lack of quantized data about this part.


References

  1. ↑ Nivière V, Vanoni MA, Zanetti G, Fontecave M. Reaction of the NAD(P)H:flavin oxidoreductase from Escherichia coli with NADPH and riboflavin: identification of intermediates. Biochemistry. 1998 Aug 25;37(34):11879-87. doi: 10.1021/bi980396f. PMID: 9718311.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 424