Difference between revisions of "Part:BBa K3799001"
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<partinfo>BBa_K3799001 short</partinfo> | <partinfo>BBa_K3799001 short</partinfo> | ||
| − | This part | + | This part encodes for IPTG inducible expression of Crispr protein,csm6.It consist of coding sequence of csm6 from Enterococcus italicus([https://parts.igem.org/Part:BBa_K3799000 BBa_K3799000]) under IPTG inducible EColi promoter Pveg([https://parts.igem.org/Part:BBa_J04500 BBa_J04500]) along with strong RBS([https://parts.igem.org/Part:BBa_B0034 BBa_B0034]) and double terminator([https://parts.igem.org/Part:BBa_B0015 BBa_B0015]). |
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===Usage and Biology=== | ===Usage and Biology=== | ||
| − | + | This part can be used to produce csm6 protein in Ecoli host under the induction of IPTG. | |
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<span class='h3bb'>Sequence and Features</span> | <span class='h3bb'>Sequence and Features</span> | ||
Latest revision as of 21:19, 21 October 2021
EiCsm6 under IPTG inducible promoter with double terminator
This part encodes for IPTG inducible expression of Crispr protein,csm6.It consist of coding sequence of csm6 from Enterococcus italicus(BBa_K3799000) under IPTG inducible EColi promoter Pveg(BBa_J04500) along with strong RBS(BBa_B0034) and double terminator(BBa_B0015).
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 534
Illegal XhoI site found at 587
Illegal XhoI site found at 1274 - 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]